An improved purification procedure for Leishmania RNA virus (LRV)

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negative...

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Detalles Bibliográficos
Autores principales: de Souza, Marcos Michel, Manzine, Livia Regina, da Silva, Marcos Vinicius G., Bettini, Jefferson, Portugal, Rodrigo Vilares, Cruz, Angela Kaysel, Arruda, Eurico, Thiemann, Otavio Henrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166301/
https://www.ncbi.nlm.nih.gov/pubmed/25242960
Descripción
Sumario:Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.

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