An improved purification procedure for Leishmania RNA virus (LRV)

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negative...

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Detalles Bibliográficos
Autores principales: de Souza, Marcos Michel, Manzine, Livia Regina, da Silva, Marcos Vinicius G., Bettini, Jefferson, Portugal, Rodrigo Vilares, Cruz, Angela Kaysel, Arruda, Eurico, Thiemann, Otavio Henrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166301/
https://www.ncbi.nlm.nih.gov/pubmed/25242960
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author de Souza, Marcos Michel
Manzine, Livia Regina
da Silva, Marcos Vinicius G.
Bettini, Jefferson
Portugal, Rodrigo Vilares
Cruz, Angela Kaysel
Arruda, Eurico
Thiemann, Otavio Henrique
author_facet de Souza, Marcos Michel
Manzine, Livia Regina
da Silva, Marcos Vinicius G.
Bettini, Jefferson
Portugal, Rodrigo Vilares
Cruz, Angela Kaysel
Arruda, Eurico
Thiemann, Otavio Henrique
author_sort de Souza, Marcos Michel
collection PubMed
description Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
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spelling pubmed-41663012014-09-29 An improved purification procedure for Leishmania RNA virus (LRV) de Souza, Marcos Michel Manzine, Livia Regina da Silva, Marcos Vinicius G. Bettini, Jefferson Portugal, Rodrigo Vilares Cruz, Angela Kaysel Arruda, Eurico Thiemann, Otavio Henrique Braz J Microbiol Genetics and Molecular Microbiology Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations. Sociedade Brasileira de Microbiologia 2014-08-29 /pmc/articles/PMC4166301/ /pubmed/25242960 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Genetics and Molecular Microbiology
de Souza, Marcos Michel
Manzine, Livia Regina
da Silva, Marcos Vinicius G.
Bettini, Jefferson
Portugal, Rodrigo Vilares
Cruz, Angela Kaysel
Arruda, Eurico
Thiemann, Otavio Henrique
An improved purification procedure for Leishmania RNA virus (LRV)
title An improved purification procedure for Leishmania RNA virus (LRV)
title_full An improved purification procedure for Leishmania RNA virus (LRV)
title_fullStr An improved purification procedure for Leishmania RNA virus (LRV)
title_full_unstemmed An improved purification procedure for Leishmania RNA virus (LRV)
title_short An improved purification procedure for Leishmania RNA virus (LRV)
title_sort improved purification procedure for leishmania rna virus (lrv)
topic Genetics and Molecular Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166301/
https://www.ncbi.nlm.nih.gov/pubmed/25242960
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