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Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development

Background. Shigella, a major diarrheal disease pathogen worldwide, is the target of vaccine development. The Global Enteric Multicenter Study (GEMS) investigated burden and etiology of moderate-to-severe diarrheal disease in children aged <60 months and matched controls without diarrhea during 3...

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Autores principales: Livio, Sofie, Strockbine, Nancy A., Panchalingam, Sandra, Tennant, Sharon M., Barry, Eileen M., Marohn, Mark E., Antonio, Martin, Hossain, Anowar, Mandomando, Inacio, Ochieng, John B., Oundo, Joseph O., Qureshi, Shahida, Ramamurthy, Thandavarayan, Tamboura, Boubou, Adegbola, Richard A., Hossain, Mohammed Jahangir, Saha, Debasish, Sen, Sunil, Faruque, Abu Syed Golam, Alonso, Pedro L., Breiman, Robert F., Zaidi, Anita K. M., Sur, Dipika, Sow, Samba O., Berkeley, Lynette Y., O'Reilly, Ciara E., Mintz, Eric D., Biswas, Kousick, Cohen, Dani, Farag, Tamer H., Nasrin, Dilruba, Wu, Yukun, Blackwelder, William C., Kotloff, Karen L., Nataro, James P., Levine, Myron M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166982/
https://www.ncbi.nlm.nih.gov/pubmed/24958238
http://dx.doi.org/10.1093/cid/ciu468
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author Livio, Sofie
Strockbine, Nancy A.
Panchalingam, Sandra
Tennant, Sharon M.
Barry, Eileen M.
Marohn, Mark E.
Antonio, Martin
Hossain, Anowar
Mandomando, Inacio
Ochieng, John B.
Oundo, Joseph O.
Qureshi, Shahida
Ramamurthy, Thandavarayan
Tamboura, Boubou
Adegbola, Richard A.
Hossain, Mohammed Jahangir
Saha, Debasish
Sen, Sunil
Faruque, Abu Syed Golam
Alonso, Pedro L.
Breiman, Robert F.
Zaidi, Anita K. M.
Sur, Dipika
Sow, Samba O.
Berkeley, Lynette Y.
O'Reilly, Ciara E.
Mintz, Eric D.
Biswas, Kousick
Cohen, Dani
Farag, Tamer H.
Nasrin, Dilruba
Wu, Yukun
Blackwelder, William C.
Kotloff, Karen L.
Nataro, James P.
Levine, Myron M.
author_facet Livio, Sofie
Strockbine, Nancy A.
Panchalingam, Sandra
Tennant, Sharon M.
Barry, Eileen M.
Marohn, Mark E.
Antonio, Martin
Hossain, Anowar
Mandomando, Inacio
Ochieng, John B.
Oundo, Joseph O.
Qureshi, Shahida
Ramamurthy, Thandavarayan
Tamboura, Boubou
Adegbola, Richard A.
Hossain, Mohammed Jahangir
Saha, Debasish
Sen, Sunil
Faruque, Abu Syed Golam
Alonso, Pedro L.
Breiman, Robert F.
Zaidi, Anita K. M.
Sur, Dipika
Sow, Samba O.
Berkeley, Lynette Y.
O'Reilly, Ciara E.
Mintz, Eric D.
Biswas, Kousick
Cohen, Dani
Farag, Tamer H.
Nasrin, Dilruba
Wu, Yukun
Blackwelder, William C.
Kotloff, Karen L.
Nataro, James P.
Levine, Myron M.
author_sort Livio, Sofie
collection PubMed
description Background. Shigella, a major diarrheal disease pathogen worldwide, is the target of vaccine development. The Global Enteric Multicenter Study (GEMS) investigated burden and etiology of moderate-to-severe diarrheal disease in children aged <60 months and matched controls without diarrhea during 3 years at 4 sites in Africa and 3 in Asia. Shigella was 1 of the 4 most common pathogens across sites and age strata. GEMS Shigella serotypes are reviewed to guide vaccine development. Methods. Subjects' stool specimens/rectal swabs were transported to site laboratories in transport media and plated onto xylose lysine desoxycholate and MacConkey agar. Suspect Shigella colonies were identified by biochemical tests and agglutination with antisera. Shigella isolates were shipped to the GEMS Reference Laboratory (Baltimore, MD) for confirmation and serotyping of S. flexneri; one-third of isolates were sent to the Centers for Disease Control and Prevention for quality control. Results. Shigella dysenteriae and S. boydii accounted for 5.0% and 5.4%, respectively, of 1130 Shigella case isolates; S. flexneri comprised 65.9% and S. sonnei 23.7%. Five serotypes/subserotypes comprised 89.4% of S. flexneri, including S. flexneri 2a, S. flexneri 6, S. flexneri 3a, S. flexneri 2b, and S. flexneri 1b. Conclusions. A broad-spectrum Shigella vaccine must protect against S. sonnei and 15 S. flexneri serotypes/subserotypes. A quadrivalent vaccine with O antigens from S. sonnei, S. flexneri 2a, S. flexneri 3a, and S. flexneri 6 can provide broad direct coverage against these most common serotypes and indirect coverage against all but 1 (rare) remaining subserotype through shared S. flexneri group antigens.
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spelling pubmed-41669822014-09-22 Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development Livio, Sofie Strockbine, Nancy A. Panchalingam, Sandra Tennant, Sharon M. Barry, Eileen M. Marohn, Mark E. Antonio, Martin Hossain, Anowar Mandomando, Inacio Ochieng, John B. Oundo, Joseph O. Qureshi, Shahida Ramamurthy, Thandavarayan Tamboura, Boubou Adegbola, Richard A. Hossain, Mohammed Jahangir Saha, Debasish Sen, Sunil Faruque, Abu Syed Golam Alonso, Pedro L. Breiman, Robert F. Zaidi, Anita K. M. Sur, Dipika Sow, Samba O. Berkeley, Lynette Y. O'Reilly, Ciara E. Mintz, Eric D. Biswas, Kousick Cohen, Dani Farag, Tamer H. Nasrin, Dilruba Wu, Yukun Blackwelder, William C. Kotloff, Karen L. Nataro, James P. Levine, Myron M. Clin Infect Dis Articles and Commentaries Background. Shigella, a major diarrheal disease pathogen worldwide, is the target of vaccine development. The Global Enteric Multicenter Study (GEMS) investigated burden and etiology of moderate-to-severe diarrheal disease in children aged <60 months and matched controls without diarrhea during 3 years at 4 sites in Africa and 3 in Asia. Shigella was 1 of the 4 most common pathogens across sites and age strata. GEMS Shigella serotypes are reviewed to guide vaccine development. Methods. Subjects' stool specimens/rectal swabs were transported to site laboratories in transport media and plated onto xylose lysine desoxycholate and MacConkey agar. Suspect Shigella colonies were identified by biochemical tests and agglutination with antisera. Shigella isolates were shipped to the GEMS Reference Laboratory (Baltimore, MD) for confirmation and serotyping of S. flexneri; one-third of isolates were sent to the Centers for Disease Control and Prevention for quality control. Results. Shigella dysenteriae and S. boydii accounted for 5.0% and 5.4%, respectively, of 1130 Shigella case isolates; S. flexneri comprised 65.9% and S. sonnei 23.7%. Five serotypes/subserotypes comprised 89.4% of S. flexneri, including S. flexneri 2a, S. flexneri 6, S. flexneri 3a, S. flexneri 2b, and S. flexneri 1b. Conclusions. A broad-spectrum Shigella vaccine must protect against S. sonnei and 15 S. flexneri serotypes/subserotypes. A quadrivalent vaccine with O antigens from S. sonnei, S. flexneri 2a, S. flexneri 3a, and S. flexneri 6 can provide broad direct coverage against these most common serotypes and indirect coverage against all but 1 (rare) remaining subserotype through shared S. flexneri group antigens. Oxford University Press 2014-10-01 2014-06-23 /pmc/articles/PMC4166982/ /pubmed/24958238 http://dx.doi.org/10.1093/cid/ciu468 Text en © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Articles and Commentaries
Livio, Sofie
Strockbine, Nancy A.
Panchalingam, Sandra
Tennant, Sharon M.
Barry, Eileen M.
Marohn, Mark E.
Antonio, Martin
Hossain, Anowar
Mandomando, Inacio
Ochieng, John B.
Oundo, Joseph O.
Qureshi, Shahida
Ramamurthy, Thandavarayan
Tamboura, Boubou
Adegbola, Richard A.
Hossain, Mohammed Jahangir
Saha, Debasish
Sen, Sunil
Faruque, Abu Syed Golam
Alonso, Pedro L.
Breiman, Robert F.
Zaidi, Anita K. M.
Sur, Dipika
Sow, Samba O.
Berkeley, Lynette Y.
O'Reilly, Ciara E.
Mintz, Eric D.
Biswas, Kousick
Cohen, Dani
Farag, Tamer H.
Nasrin, Dilruba
Wu, Yukun
Blackwelder, William C.
Kotloff, Karen L.
Nataro, James P.
Levine, Myron M.
Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title_full Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title_fullStr Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title_full_unstemmed Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title_short Shigella Isolates From the Global Enteric Multicenter Study Inform Vaccine Development
title_sort shigella isolates from the global enteric multicenter study inform vaccine development
topic Articles and Commentaries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166982/
https://www.ncbi.nlm.nih.gov/pubmed/24958238
http://dx.doi.org/10.1093/cid/ciu468
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