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Apoptosis in HEp-2 cells infected with Ureaplasma diversum
BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location of Ureaplasma diversum may trigger disturbances in the host cell. This includes a...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167145/ https://www.ncbi.nlm.nih.gov/pubmed/25299837 http://dx.doi.org/10.1186/0717-6287-47-38 |
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author | Amorim, Aline Teixeira Marques, Lucas Miranda Santos, Angelita Maria Oliveira Gusmão Martins, Hellen Braga Barbosa, Maysa Santos Rezende, Izadora Souza Andrade, Ewerton Ferraz Campos, Guilherme Barreto Lobão, Tássia Neves Cortez, Beatriz Araujo Monezi, Telma Alvez Machado-Santelli, Glaucia Maria Timenetsky, Jorge |
author_facet | Amorim, Aline Teixeira Marques, Lucas Miranda Santos, Angelita Maria Oliveira Gusmão Martins, Hellen Braga Barbosa, Maysa Santos Rezende, Izadora Souza Andrade, Ewerton Ferraz Campos, Guilherme Barreto Lobão, Tássia Neves Cortez, Beatriz Araujo Monezi, Telma Alvez Machado-Santelli, Glaucia Maria Timenetsky, Jorge |
author_sort | Amorim, Aline Teixeira |
collection | PubMed |
description | BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location of Ureaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversum invasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells. |
format | Online Article Text |
id | pubmed-4167145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41671452014-09-19 Apoptosis in HEp-2 cells infected with Ureaplasma diversum Amorim, Aline Teixeira Marques, Lucas Miranda Santos, Angelita Maria Oliveira Gusmão Martins, Hellen Braga Barbosa, Maysa Santos Rezende, Izadora Souza Andrade, Ewerton Ferraz Campos, Guilherme Barreto Lobão, Tássia Neves Cortez, Beatriz Araujo Monezi, Telma Alvez Machado-Santelli, Glaucia Maria Timenetsky, Jorge Biol Res Research Article BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location of Ureaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversum invasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells. BioMed Central 2014-09-04 /pmc/articles/PMC4167145/ /pubmed/25299837 http://dx.doi.org/10.1186/0717-6287-47-38 Text en © Amorim et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Amorim, Aline Teixeira Marques, Lucas Miranda Santos, Angelita Maria Oliveira Gusmão Martins, Hellen Braga Barbosa, Maysa Santos Rezende, Izadora Souza Andrade, Ewerton Ferraz Campos, Guilherme Barreto Lobão, Tássia Neves Cortez, Beatriz Araujo Monezi, Telma Alvez Machado-Santelli, Glaucia Maria Timenetsky, Jorge Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title | Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title_full | Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title_fullStr | Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title_full_unstemmed | Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title_short | Apoptosis in HEp-2 cells infected with Ureaplasma diversum |
title_sort | apoptosis in hep-2 cells infected with ureaplasma diversum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167145/ https://www.ncbi.nlm.nih.gov/pubmed/25299837 http://dx.doi.org/10.1186/0717-6287-47-38 |
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