Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes...

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Detalles Bibliográficos
Autores principales: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167323/
https://www.ncbi.nlm.nih.gov/pubmed/25226542
http://dx.doi.org/10.1371/journal.pone.0107425
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author Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
author_facet Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
author_sort Basu, Reelina
collection PubMed
description Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.
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spelling pubmed-41673232014-09-22 Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH Basu, Reelina Lai, Lan-Tian Meng, Zhenyu Wu, Jun Shao, Fangwei Zhang, Li-Feng PLoS One Research Article Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts. Public Library of Science 2014-09-16 /pmc/articles/PMC4167323/ /pubmed/25226542 http://dx.doi.org/10.1371/journal.pone.0107425 Text en © 2014 Basu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Basu, Reelina
Lai, Lan-Tian
Meng, Zhenyu
Wu, Jun
Shao, Fangwei
Zhang, Li-Feng
Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title_full Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title_fullStr Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title_full_unstemmed Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title_short Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
title_sort using amino-labeled nucleotide probes for simultaneous single molecule rna-dna fish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167323/
https://www.ncbi.nlm.nih.gov/pubmed/25226542
http://dx.doi.org/10.1371/journal.pone.0107425
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