Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens

We report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enteri...

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Detalles Bibliográficos
Autores principales: Kersting, Sebastian, Rausch, Valentina, Bier, Frank F., von Nickisch-Rosenegk, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4167443/
https://www.ncbi.nlm.nih.gov/pubmed/25253912
http://dx.doi.org/10.1007/s00604-014-1198-5

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