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New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells

Muscle regeneration involves the activation of satellite cells, is regulated at the genetic and epigenetic levels, and is strongly influenced by gene activation and environmental conditions. The aim of this study was to determine whether the overexpression of mIGF-1 can modify functional features of...

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Autores principales: Guarnieri, Simone, Morabito, Caterina, Belia, Silvia, Barberi, Laura, Musarò, Antonio, Fanò-Illic, Giorgio, Mariggiò, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168228/
https://www.ncbi.nlm.nih.gov/pubmed/25229238
http://dx.doi.org/10.1371/journal.pone.0107753
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author Guarnieri, Simone
Morabito, Caterina
Belia, Silvia
Barberi, Laura
Musarò, Antonio
Fanò-Illic, Giorgio
Mariggiò, Maria A.
author_facet Guarnieri, Simone
Morabito, Caterina
Belia, Silvia
Barberi, Laura
Musarò, Antonio
Fanò-Illic, Giorgio
Mariggiò, Maria A.
author_sort Guarnieri, Simone
collection PubMed
description Muscle regeneration involves the activation of satellite cells, is regulated at the genetic and epigenetic levels, and is strongly influenced by gene activation and environmental conditions. The aim of this study was to determine whether the overexpression of mIGF-1 can modify functional features of satellite cells during the differentiation process, particularly in relation to modifications of intracellular Ca(2+) handling. Satellite cells were isolated from wild-type and MLC/mIGF-1 transgenic mice. The cells were differentiated in vitro, and morphological analyses, intracellular Ca(2+) measurements, and ionic current recordings were performed. mIGF-1 overexpression accelerates satellite cell differentiation and promotes myotube hypertrophy. In addition, mIGF-1 overexpression-induced potentiation of myogenesis triggers both quantitative and qualitative changes to the control of intracellular Ca(2+) handling. In particular, the differentiated MLC/mIGF-1 transgenic myotubes have reduced velocity and amplitude of intracellular Ca(2+) increases after stimulation with caffeine, KCl and acetylcholine. This appears to be due, at least in part, to changes in the physico-chemical state of the sarcolemma (increased membrane lipid oxidation, increased output currents) and to increased expression of dihydropyridine voltage-operated Ca(2+) channels. Interestingly, extracellular ATP and GTP evoke intracellular Ca(2+) mobilization to greater extents in the MLC/mIGF-1 transgenic satellite cells, compared to the wild-type cells. These data suggest that these MLC/mIGF-1 transgenic satellite cells are more sensitive to trophic stimuli, which can potentiate the effects of mIGF-1 on the myogenic programme.
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spelling pubmed-41682282014-09-22 New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells Guarnieri, Simone Morabito, Caterina Belia, Silvia Barberi, Laura Musarò, Antonio Fanò-Illic, Giorgio Mariggiò, Maria A. PLoS One Research Article Muscle regeneration involves the activation of satellite cells, is regulated at the genetic and epigenetic levels, and is strongly influenced by gene activation and environmental conditions. The aim of this study was to determine whether the overexpression of mIGF-1 can modify functional features of satellite cells during the differentiation process, particularly in relation to modifications of intracellular Ca(2+) handling. Satellite cells were isolated from wild-type and MLC/mIGF-1 transgenic mice. The cells were differentiated in vitro, and morphological analyses, intracellular Ca(2+) measurements, and ionic current recordings were performed. mIGF-1 overexpression accelerates satellite cell differentiation and promotes myotube hypertrophy. In addition, mIGF-1 overexpression-induced potentiation of myogenesis triggers both quantitative and qualitative changes to the control of intracellular Ca(2+) handling. In particular, the differentiated MLC/mIGF-1 transgenic myotubes have reduced velocity and amplitude of intracellular Ca(2+) increases after stimulation with caffeine, KCl and acetylcholine. This appears to be due, at least in part, to changes in the physico-chemical state of the sarcolemma (increased membrane lipid oxidation, increased output currents) and to increased expression of dihydropyridine voltage-operated Ca(2+) channels. Interestingly, extracellular ATP and GTP evoke intracellular Ca(2+) mobilization to greater extents in the MLC/mIGF-1 transgenic satellite cells, compared to the wild-type cells. These data suggest that these MLC/mIGF-1 transgenic satellite cells are more sensitive to trophic stimuli, which can potentiate the effects of mIGF-1 on the myogenic programme. Public Library of Science 2014-09-17 /pmc/articles/PMC4168228/ /pubmed/25229238 http://dx.doi.org/10.1371/journal.pone.0107753 Text en © 2014 Guarnieri et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Guarnieri, Simone
Morabito, Caterina
Belia, Silvia
Barberi, Laura
Musarò, Antonio
Fanò-Illic, Giorgio
Mariggiò, Maria A.
New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title_full New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title_fullStr New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title_full_unstemmed New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title_short New Insights into the Relationship between mIGF-1-Induced Hypertrophy and Ca(2+) Handling in Differentiated Satellite Cells
title_sort new insights into the relationship between migf-1-induced hypertrophy and ca(2+) handling in differentiated satellite cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168228/
https://www.ncbi.nlm.nih.gov/pubmed/25229238
http://dx.doi.org/10.1371/journal.pone.0107753
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