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An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partial...

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Detalles Bibliográficos
Autores principales: Pilehvar, Sanaz, Dierckx, Tarryn, Blust, Ronny, Breugelmans, Tom, De Wael, Karolien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168455/
https://www.ncbi.nlm.nih.gov/pubmed/25004156
http://dx.doi.org/10.3390/s140712059
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author Pilehvar, Sanaz
Dierckx, Tarryn
Blust, Ronny
Breugelmans, Tom
De Wael, Karolien
author_facet Pilehvar, Sanaz
Dierckx, Tarryn
Blust, Ronny
Breugelmans, Tom
De Wael, Karolien
author_sort Pilehvar, Sanaz
collection PubMed
description We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.
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spelling pubmed-41684552014-09-19 An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol Pilehvar, Sanaz Dierckx, Tarryn Blust, Ronny Breugelmans, Tom De Wael, Karolien Sensors (Basel) Article We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP. MDPI 2014-07-07 /pmc/articles/PMC4168455/ /pubmed/25004156 http://dx.doi.org/10.3390/s140712059 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pilehvar, Sanaz
Dierckx, Tarryn
Blust, Ronny
Breugelmans, Tom
De Wael, Karolien
An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_full An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_fullStr An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_full_unstemmed An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_short An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol
title_sort electrochemical impedimetric aptasensing platform for sensitive and selective detection of small molecules such as chloramphenicol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168455/
https://www.ncbi.nlm.nih.gov/pubmed/25004156
http://dx.doi.org/10.3390/s140712059
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