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In Vitro Evaluation of Fluorescence Glucose Biosensor Response

Rapid, accurate, and minimally-invasive glucose biosensors based on Förster Resonance Energy Transfer (FRET) for glucose measurement have the potential to enhance diabetes control. However, a standard set of in vitro approaches for evaluating optical glucose biosensor response under controlled condi...

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Autores principales: Aloraefy, Mamdouh, Pfefer, T. Joshua, Ramella-Roman, Jessica C., Sapsford, Kim E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168472/
https://www.ncbi.nlm.nih.gov/pubmed/25006996
http://dx.doi.org/10.3390/s140712127
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author Aloraefy, Mamdouh
Pfefer, T. Joshua
Ramella-Roman, Jessica C.
Sapsford, Kim E.
author_facet Aloraefy, Mamdouh
Pfefer, T. Joshua
Ramella-Roman, Jessica C.
Sapsford, Kim E.
author_sort Aloraefy, Mamdouh
collection PubMed
description Rapid, accurate, and minimally-invasive glucose biosensors based on Förster Resonance Energy Transfer (FRET) for glucose measurement have the potential to enhance diabetes control. However, a standard set of in vitro approaches for evaluating optical glucose biosensor response under controlled conditions would facilitate technological innovation and clinical translation. Towards this end, we have identified key characteristics and response test methods, fabricated FRET-based glucose biosensors, and characterized biosensor performance using these test methods. The biosensors were based on competitive binding between dextran and glucose to concanavalin A and incorporated long-wavelength fluorescence dye pairs. Testing characteristics included spectral response, linearity, sensitivity, limit of detection, kinetic response, reversibility, stability, precision, and accuracy. The biosensor demonstrated a fluorescence change of 45% in the presence of 400 mg/dL glucose, a mean absolute relative difference of less than 11%, a limit of detection of 25 mg/dL, a response time of 15 min, and a decay in fluorescence intensity of 72% over 30 days. The battery of tests presented here for objective, quantitative in vitro evaluation of FRET glucose biosensors performance have the potential to form the basis of future consensus standards. By implementing these test methods for a long-visible-wavelength biosensor, we were able to demonstrate strengths and weaknesses with a new level of thoroughness and rigor.
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spelling pubmed-41684722014-09-19 In Vitro Evaluation of Fluorescence Glucose Biosensor Response Aloraefy, Mamdouh Pfefer, T. Joshua Ramella-Roman, Jessica C. Sapsford, Kim E. Sensors (Basel) Article Rapid, accurate, and minimally-invasive glucose biosensors based on Förster Resonance Energy Transfer (FRET) for glucose measurement have the potential to enhance diabetes control. However, a standard set of in vitro approaches for evaluating optical glucose biosensor response under controlled conditions would facilitate technological innovation and clinical translation. Towards this end, we have identified key characteristics and response test methods, fabricated FRET-based glucose biosensors, and characterized biosensor performance using these test methods. The biosensors were based on competitive binding between dextran and glucose to concanavalin A and incorporated long-wavelength fluorescence dye pairs. Testing characteristics included spectral response, linearity, sensitivity, limit of detection, kinetic response, reversibility, stability, precision, and accuracy. The biosensor demonstrated a fluorescence change of 45% in the presence of 400 mg/dL glucose, a mean absolute relative difference of less than 11%, a limit of detection of 25 mg/dL, a response time of 15 min, and a decay in fluorescence intensity of 72% over 30 days. The battery of tests presented here for objective, quantitative in vitro evaluation of FRET glucose biosensors performance have the potential to form the basis of future consensus standards. By implementing these test methods for a long-visible-wavelength biosensor, we were able to demonstrate strengths and weaknesses with a new level of thoroughness and rigor. MDPI 2014-07-08 /pmc/articles/PMC4168472/ /pubmed/25006996 http://dx.doi.org/10.3390/s140712127 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Aloraefy, Mamdouh
Pfefer, T. Joshua
Ramella-Roman, Jessica C.
Sapsford, Kim E.
In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title_full In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title_fullStr In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title_full_unstemmed In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title_short In Vitro Evaluation of Fluorescence Glucose Biosensor Response
title_sort in vitro evaluation of fluorescence glucose biosensor response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168472/
https://www.ncbi.nlm.nih.gov/pubmed/25006996
http://dx.doi.org/10.3390/s140712127
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