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A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs

Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable bi...

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Autores principales: Philibert, Robert A, Penaluna, Brandan, White, Teresa, Shires, Sarah, Gunter, Tracy, Liesveld, Jill, Erwin, Cheryl, Hollenbeck, Nancy, Osborn, Terry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169013/
https://www.ncbi.nlm.nih.gov/pubmed/25147915
http://dx.doi.org/10.4161/epi.32252
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author Philibert, Robert A
Penaluna, Brandan
White, Teresa
Shires, Sarah
Gunter, Tracy
Liesveld, Jill
Erwin, Cheryl
Hollenbeck, Nancy
Osborn, Terry
author_facet Philibert, Robert A
Penaluna, Brandan
White, Teresa
Shires, Sarah
Gunter, Tracy
Liesveld, Jill
Erwin, Cheryl
Hollenbeck, Nancy
Osborn, Terry
author_sort Philibert, Robert A
collection PubMed
description Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response.
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spelling pubmed-41690132015-09-01 A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs Philibert, Robert A Penaluna, Brandan White, Teresa Shires, Sarah Gunter, Tracy Liesveld, Jill Erwin, Cheryl Hollenbeck, Nancy Osborn, Terry Epigenetics Brief Report Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response. Landes Bioscience 2014-09-01 2014-08-11 /pmc/articles/PMC4169013/ /pubmed/25147915 http://dx.doi.org/10.4161/epi.32252 Text en Copyright © 2014 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Brief Report
Philibert, Robert A
Penaluna, Brandan
White, Teresa
Shires, Sarah
Gunter, Tracy
Liesveld, Jill
Erwin, Cheryl
Hollenbeck, Nancy
Osborn, Terry
A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title_full A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title_fullStr A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title_full_unstemmed A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title_short A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
title_sort pilot examination of the genome-wide dna methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169013/
https://www.ncbi.nlm.nih.gov/pubmed/25147915
http://dx.doi.org/10.4161/epi.32252
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