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A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable bi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169013/ https://www.ncbi.nlm.nih.gov/pubmed/25147915 http://dx.doi.org/10.4161/epi.32252 |
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author | Philibert, Robert A Penaluna, Brandan White, Teresa Shires, Sarah Gunter, Tracy Liesveld, Jill Erwin, Cheryl Hollenbeck, Nancy Osborn, Terry |
author_facet | Philibert, Robert A Penaluna, Brandan White, Teresa Shires, Sarah Gunter, Tracy Liesveld, Jill Erwin, Cheryl Hollenbeck, Nancy Osborn, Terry |
author_sort | Philibert, Robert A |
collection | PubMed |
description | Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response. |
format | Online Article Text |
id | pubmed-4169013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-41690132015-09-01 A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs Philibert, Robert A Penaluna, Brandan White, Teresa Shires, Sarah Gunter, Tracy Liesveld, Jill Erwin, Cheryl Hollenbeck, Nancy Osborn, Terry Epigenetics Brief Report Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response. Landes Bioscience 2014-09-01 2014-08-11 /pmc/articles/PMC4169013/ /pubmed/25147915 http://dx.doi.org/10.4161/epi.32252 Text en Copyright © 2014 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Brief Report Philibert, Robert A Penaluna, Brandan White, Teresa Shires, Sarah Gunter, Tracy Liesveld, Jill Erwin, Cheryl Hollenbeck, Nancy Osborn, Terry A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title | A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title_full | A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title_fullStr | A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title_full_unstemmed | A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title_short | A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
title_sort | pilot examination of the genome-wide dna methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169013/ https://www.ncbi.nlm.nih.gov/pubmed/25147915 http://dx.doi.org/10.4161/epi.32252 |
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