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Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
BACKGROUND: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T....
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169256/ https://www.ncbi.nlm.nih.gov/pubmed/25233456 http://dx.doi.org/10.1371/journal.pntd.0003176 |
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author | Stoco, Patrícia Hermes Wagner, Glauber Talavera-Lopez, Carlos Gerber, Alexandra Zaha, Arnaldo Thompson, Claudia Elizabeth Bartholomeu, Daniella Castanheira Lückemeyer, Débora Denardin Bahia, Diana Loreto, Elgion Prestes, Elisa Beatriz Lima, Fábio Mitsuo Rodrigues-Luiz, Gabriela Vallejo, Gustavo Adolfo Filho, José Franco da Silveira Schenkman, Sérgio Monteiro, Karina Mariante Tyler, Kevin Morris de Almeida, Luiz Gonzaga Paula Ortiz, Mauro Freitas Chiurillo, Miguel Angel de Moraes, Milene Höehr Cunha, Oberdan de Lima Mendonça-Neto, Rondon Silva, Rosane Teixeira, Santuza Maria Ribeiro Murta, Silvane Maria Fonseca Sincero, Thais Cristine Marques Mendes, Tiago Antonio de Oliveira Urmenyi, Turán Peter Silva, Viviane Grazielle DaRocha, Wanderson Duarte Andersson, Björn Romanha, Álvaro José Steindel, Mário de Vasconcelos, Ana Tereza Ribeiro Grisard, Edmundo Carlos |
author_facet | Stoco, Patrícia Hermes Wagner, Glauber Talavera-Lopez, Carlos Gerber, Alexandra Zaha, Arnaldo Thompson, Claudia Elizabeth Bartholomeu, Daniella Castanheira Lückemeyer, Débora Denardin Bahia, Diana Loreto, Elgion Prestes, Elisa Beatriz Lima, Fábio Mitsuo Rodrigues-Luiz, Gabriela Vallejo, Gustavo Adolfo Filho, José Franco da Silveira Schenkman, Sérgio Monteiro, Karina Mariante Tyler, Kevin Morris de Almeida, Luiz Gonzaga Paula Ortiz, Mauro Freitas Chiurillo, Miguel Angel de Moraes, Milene Höehr Cunha, Oberdan de Lima Mendonça-Neto, Rondon Silva, Rosane Teixeira, Santuza Maria Ribeiro Murta, Silvane Maria Fonseca Sincero, Thais Cristine Marques Mendes, Tiago Antonio de Oliveira Urmenyi, Turán Peter Silva, Viviane Grazielle DaRocha, Wanderson Duarte Andersson, Björn Romanha, Álvaro José Steindel, Mário de Vasconcelos, Ana Tereza Ribeiro Grisard, Edmundo Carlos |
author_sort | Stoco, Patrícia Hermes |
collection | PubMed |
description | BACKGROUND: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts. METHODOLOGY/PRINCIPAL FINDINGS: The T. rangeli haploid genome is ∼24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heat-shock proteins. CONCLUSIONS/SIGNIFICANCE: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets. |
format | Online Article Text |
id | pubmed-4169256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41692562014-09-22 Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli Stoco, Patrícia Hermes Wagner, Glauber Talavera-Lopez, Carlos Gerber, Alexandra Zaha, Arnaldo Thompson, Claudia Elizabeth Bartholomeu, Daniella Castanheira Lückemeyer, Débora Denardin Bahia, Diana Loreto, Elgion Prestes, Elisa Beatriz Lima, Fábio Mitsuo Rodrigues-Luiz, Gabriela Vallejo, Gustavo Adolfo Filho, José Franco da Silveira Schenkman, Sérgio Monteiro, Karina Mariante Tyler, Kevin Morris de Almeida, Luiz Gonzaga Paula Ortiz, Mauro Freitas Chiurillo, Miguel Angel de Moraes, Milene Höehr Cunha, Oberdan de Lima Mendonça-Neto, Rondon Silva, Rosane Teixeira, Santuza Maria Ribeiro Murta, Silvane Maria Fonseca Sincero, Thais Cristine Marques Mendes, Tiago Antonio de Oliveira Urmenyi, Turán Peter Silva, Viviane Grazielle DaRocha, Wanderson Duarte Andersson, Björn Romanha, Álvaro José Steindel, Mário de Vasconcelos, Ana Tereza Ribeiro Grisard, Edmundo Carlos PLoS Negl Trop Dis Research Article BACKGROUND: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts. METHODOLOGY/PRINCIPAL FINDINGS: The T. rangeli haploid genome is ∼24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heat-shock proteins. CONCLUSIONS/SIGNIFICANCE: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets. Public Library of Science 2014-09-18 /pmc/articles/PMC4169256/ /pubmed/25233456 http://dx.doi.org/10.1371/journal.pntd.0003176 Text en © 2014 Stoco et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Stoco, Patrícia Hermes Wagner, Glauber Talavera-Lopez, Carlos Gerber, Alexandra Zaha, Arnaldo Thompson, Claudia Elizabeth Bartholomeu, Daniella Castanheira Lückemeyer, Débora Denardin Bahia, Diana Loreto, Elgion Prestes, Elisa Beatriz Lima, Fábio Mitsuo Rodrigues-Luiz, Gabriela Vallejo, Gustavo Adolfo Filho, José Franco da Silveira Schenkman, Sérgio Monteiro, Karina Mariante Tyler, Kevin Morris de Almeida, Luiz Gonzaga Paula Ortiz, Mauro Freitas Chiurillo, Miguel Angel de Moraes, Milene Höehr Cunha, Oberdan de Lima Mendonça-Neto, Rondon Silva, Rosane Teixeira, Santuza Maria Ribeiro Murta, Silvane Maria Fonseca Sincero, Thais Cristine Marques Mendes, Tiago Antonio de Oliveira Urmenyi, Turán Peter Silva, Viviane Grazielle DaRocha, Wanderson Duarte Andersson, Björn Romanha, Álvaro José Steindel, Mário de Vasconcelos, Ana Tereza Ribeiro Grisard, Edmundo Carlos Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli |
title | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
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title_full | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
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title_fullStr | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
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title_full_unstemmed | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
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title_short | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
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title_sort | genome of the avirulent human-infective trypanosome—trypanosoma rangeli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169256/ https://www.ncbi.nlm.nih.gov/pubmed/25233456 http://dx.doi.org/10.1371/journal.pntd.0003176 |
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