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Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway

Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (At...

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Autores principales: Fujikura, Ushio, Elsaesser, Lore, Breuninger, Holger, Sánchez-Rodríguez, Clara, Ivakov, Alexander, Laux, Thomas, Findlay, Kim, Persson, Staffan, Lenhard, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169273/
https://www.ncbi.nlm.nih.gov/pubmed/25232944
http://dx.doi.org/10.1371/journal.pgen.1004627
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author Fujikura, Ushio
Elsaesser, Lore
Breuninger, Holger
Sánchez-Rodríguez, Clara
Ivakov, Alexander
Laux, Thomas
Findlay, Kim
Persson, Staffan
Lenhard, Michael
author_facet Fujikura, Ushio
Elsaesser, Lore
Breuninger, Holger
Sánchez-Rodríguez, Clara
Ivakov, Alexander
Laux, Thomas
Findlay, Kim
Persson, Staffan
Lenhard, Michael
author_sort Fujikura, Ushio
collection PubMed
description Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in Arabidopsis thaliana, with loss-of-function atkin13a mutants forming larger petals with larger cells. The homolog, AtKINESIN-13B, also affects cell expansion and double mutants display growth, gametophytic and early embryonic defects, indicating a redundant role of the two genes. AtKIN13A is known to depolymerize microtubules and influence Golgi motility and distribution. Consistent with this function, AtKIN13A interacts genetically with ANGUSTIFOLIA, encoding a regulator of Golgi dynamics. Reduced AtKIN13A activity alters cell wall structure as assessed by Fourier-transformed infrared-spectroscopy and triggers signalling via the THESEUS1-dependent cell-wall integrity pathway, which in turn promotes the excess cell expansion in the atkin13a mutant. Thus, our results indicate that the intracellular activity of AtKIN13A regulates cell expansion and wall architecture via THESEUS1, providing a compelling case of interplay between cell wall integrity sensing and expansion.
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spelling pubmed-41692732014-09-22 Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway Fujikura, Ushio Elsaesser, Lore Breuninger, Holger Sánchez-Rodríguez, Clara Ivakov, Alexander Laux, Thomas Findlay, Kim Persson, Staffan Lenhard, Michael PLoS Genet Research Article Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in Arabidopsis thaliana, with loss-of-function atkin13a mutants forming larger petals with larger cells. The homolog, AtKINESIN-13B, also affects cell expansion and double mutants display growth, gametophytic and early embryonic defects, indicating a redundant role of the two genes. AtKIN13A is known to depolymerize microtubules and influence Golgi motility and distribution. Consistent with this function, AtKIN13A interacts genetically with ANGUSTIFOLIA, encoding a regulator of Golgi dynamics. Reduced AtKIN13A activity alters cell wall structure as assessed by Fourier-transformed infrared-spectroscopy and triggers signalling via the THESEUS1-dependent cell-wall integrity pathway, which in turn promotes the excess cell expansion in the atkin13a mutant. Thus, our results indicate that the intracellular activity of AtKIN13A regulates cell expansion and wall architecture via THESEUS1, providing a compelling case of interplay between cell wall integrity sensing and expansion. Public Library of Science 2014-09-18 /pmc/articles/PMC4169273/ /pubmed/25232944 http://dx.doi.org/10.1371/journal.pgen.1004627 Text en © 2014 Fujikura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fujikura, Ushio
Elsaesser, Lore
Breuninger, Holger
Sánchez-Rodríguez, Clara
Ivakov, Alexander
Laux, Thomas
Findlay, Kim
Persson, Staffan
Lenhard, Michael
Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title_full Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title_fullStr Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title_full_unstemmed Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title_short Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway
title_sort atkinesin-13a modulates cell-wall synthesis and cell expansion in arabidopsis thaliana via the theseus1 pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169273/
https://www.ncbi.nlm.nih.gov/pubmed/25232944
http://dx.doi.org/10.1371/journal.pgen.1004627
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