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Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection
Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169399/ https://www.ncbi.nlm.nih.gov/pubmed/25232736 http://dx.doi.org/10.1371/journal.pone.0107153 |
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author | Curtis, Kelly A. Ambrose, Krystin M. Kennedy, M. Susan Owen, S. Michele |
author_facet | Curtis, Kelly A. Ambrose, Krystin M. Kennedy, M. Susan Owen, S. Michele |
author_sort | Curtis, Kelly A. |
collection | PubMed |
description | Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample source for HIV surveillance, given the simplified and cost-effective methods of specimen collection, storage, and shipment. Here, we evaluated the use of DBS in conjunction with an in-house multiplex TRI, the HIV-1-specific Bio-Plex assay, which measures direct antibody binding and avidity to multiple HIV-1 analytes. The assay performance was comparable between matched plasma and DBS samples from HIV-1 infected individuals obtained from diverse sources. The coefficients of variation, comparing the median antibody reactivity for each analyte between plasma and DBS, ranged from 2.78% to 9.40% and the correlation coefficients between the two sample types ranged from 0.89 to 0.97, depending on the analyte. The correlation in antibody reactivity between laboratory and site-prepared DBS for each analyte ranged from 0.87 to 0.98 and from 0.90 to 0.97 between site-prepared DBS and plasma. The correlation in assay measures between plasma and DBS indicate that the sample types can be used interchangeably with the Bio-Plex format, without negatively impacting the misclassification rate of the assay. |
format | Online Article Text |
id | pubmed-4169399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41693992014-09-22 Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection Curtis, Kelly A. Ambrose, Krystin M. Kennedy, M. Susan Owen, S. Michele PLoS One Research Article Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample source for HIV surveillance, given the simplified and cost-effective methods of specimen collection, storage, and shipment. Here, we evaluated the use of DBS in conjunction with an in-house multiplex TRI, the HIV-1-specific Bio-Plex assay, which measures direct antibody binding and avidity to multiple HIV-1 analytes. The assay performance was comparable between matched plasma and DBS samples from HIV-1 infected individuals obtained from diverse sources. The coefficients of variation, comparing the median antibody reactivity for each analyte between plasma and DBS, ranged from 2.78% to 9.40% and the correlation coefficients between the two sample types ranged from 0.89 to 0.97, depending on the analyte. The correlation in antibody reactivity between laboratory and site-prepared DBS for each analyte ranged from 0.87 to 0.98 and from 0.90 to 0.97 between site-prepared DBS and plasma. The correlation in assay measures between plasma and DBS indicate that the sample types can be used interchangeably with the Bio-Plex format, without negatively impacting the misclassification rate of the assay. Public Library of Science 2014-09-18 /pmc/articles/PMC4169399/ /pubmed/25232736 http://dx.doi.org/10.1371/journal.pone.0107153 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Curtis, Kelly A. Ambrose, Krystin M. Kennedy, M. Susan Owen, S. Michele Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title | Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title_full | Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title_fullStr | Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title_full_unstemmed | Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title_short | Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection |
title_sort | evaluation of dried blood spots with a multiplex assay for measuring recent hiv-1 infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169399/ https://www.ncbi.nlm.nih.gov/pubmed/25232736 http://dx.doi.org/10.1371/journal.pone.0107153 |
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