Atomic Resolution Structure of a Protein Prepared by Non-Enzymatic His-Tag Removal. Crystallographic and NMR Study of GmSPI-2 Inhibitor

Purification of suitable quantity of homogenous protein is very often the bottleneck in protein structural studies. Overexpression of a desired gene and attachment of enzymatically cleavable affinity tags to the protein of interest made a breakthrough in this field. Here we describe the structure of...

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Detalles Bibliográficos
Autores principales: Kopera, Edyta, Bal, Wojciech, Lenarčič Živkovič, Martina, Dvornyk, Angela, Kludkiewicz, Barbara, Grzelak, Krystyna, Zhukov, Igor, Zagórski-Ostoja, Włodzimierz, Jaskolski, Mariusz, Krzywda, Szymon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169406/
https://www.ncbi.nlm.nih.gov/pubmed/25233114
http://dx.doi.org/10.1371/journal.pone.0106936

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