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miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study

Relative to western populations, the percentage of women diagnosed with breast cancer at a young age in Lebanon is high. While the younger age of the Lebanese population compared to the West certainly contributes to this difference, potential genetic, reproductive and/or biological factors likely pl...

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Autores principales: Nassar, Farah J., El Sabban, Maya, Zgheib, Nathalie K., Tfayli, Arafat, Boulos, Fouad, Jabbour, Mark, Saghir, Nagi S. E. l., Talhouk, Rabih, Bazarbachi, Ali, Calin, George A., Nasr, Rihab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169426/
https://www.ncbi.nlm.nih.gov/pubmed/25232827
http://dx.doi.org/10.1371/journal.pone.0107566
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author Nassar, Farah J.
El Sabban, Maya
Zgheib, Nathalie K.
Tfayli, Arafat
Boulos, Fouad
Jabbour, Mark
Saghir, Nagi S. E. l.
Talhouk, Rabih
Bazarbachi, Ali
Calin, George A.
Nasr, Rihab
author_facet Nassar, Farah J.
El Sabban, Maya
Zgheib, Nathalie K.
Tfayli, Arafat
Boulos, Fouad
Jabbour, Mark
Saghir, Nagi S. E. l.
Talhouk, Rabih
Bazarbachi, Ali
Calin, George A.
Nasr, Rihab
author_sort Nassar, Farah J.
collection PubMed
description Relative to western populations, the percentage of women diagnosed with breast cancer at a young age in Lebanon is high. While the younger age of the Lebanese population compared to the West certainly contributes to this difference, potential genetic, reproductive and/or biological factors likely play an important role. The objective of this study is to investigate the contribution of miRNAs in this setting through the analysis of the expression of five reported dysregulated miRNAs, miR-148b, miR-10b, miR-21, miR-221, and miR-155 in 20 normal and 57 cancerous breast tissues from Lebanese breast cancer patients. After finding their relative expression by quantitative reverse transcription real time PCR, the results were analyzed with respect to the patients’ clinical and histopathology presentations. Compared to normal breast tissues, significant upregulation of miR-155, miR-21 and miR-148b, notable downregulation of miR-10b and non-significant expression of miR-221 were observed in tumor tissues. Moreover, miR-10b was significantly underexpressed in estrogen/progesterone receptor (ER/PR) negative tumors relative to ER/PR positive tumor tissues. miR-155 was also significantly overexpressed in postmenopausal patients and in those of age at diagnosis greater than 40 years old as well as in PR negative or in human epidermal growth factor 2 (Her2) positive tissues. This study is the first one to report miRNA expression patterns in Lebanese breast cancer patients. We found that differential miRNA expression in breast cancer could be variable between Lebanese and Western populations. miR-10b was positively correlated with the ER and PR status and miR-155 could be a noteworthy biomarker for the menopausal state, age at diagnosis, PR and Her2 status. Hence, miRNA can be used as biomarkers for early breast cancer detection.
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spelling pubmed-41694262014-09-22 miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study Nassar, Farah J. El Sabban, Maya Zgheib, Nathalie K. Tfayli, Arafat Boulos, Fouad Jabbour, Mark Saghir, Nagi S. E. l. Talhouk, Rabih Bazarbachi, Ali Calin, George A. Nasr, Rihab PLoS One Research Article Relative to western populations, the percentage of women diagnosed with breast cancer at a young age in Lebanon is high. While the younger age of the Lebanese population compared to the West certainly contributes to this difference, potential genetic, reproductive and/or biological factors likely play an important role. The objective of this study is to investigate the contribution of miRNAs in this setting through the analysis of the expression of five reported dysregulated miRNAs, miR-148b, miR-10b, miR-21, miR-221, and miR-155 in 20 normal and 57 cancerous breast tissues from Lebanese breast cancer patients. After finding their relative expression by quantitative reverse transcription real time PCR, the results were analyzed with respect to the patients’ clinical and histopathology presentations. Compared to normal breast tissues, significant upregulation of miR-155, miR-21 and miR-148b, notable downregulation of miR-10b and non-significant expression of miR-221 were observed in tumor tissues. Moreover, miR-10b was significantly underexpressed in estrogen/progesterone receptor (ER/PR) negative tumors relative to ER/PR positive tumor tissues. miR-155 was also significantly overexpressed in postmenopausal patients and in those of age at diagnosis greater than 40 years old as well as in PR negative or in human epidermal growth factor 2 (Her2) positive tissues. This study is the first one to report miRNA expression patterns in Lebanese breast cancer patients. We found that differential miRNA expression in breast cancer could be variable between Lebanese and Western populations. miR-10b was positively correlated with the ER and PR status and miR-155 could be a noteworthy biomarker for the menopausal state, age at diagnosis, PR and Her2 status. Hence, miRNA can be used as biomarkers for early breast cancer detection. Public Library of Science 2014-09-18 /pmc/articles/PMC4169426/ /pubmed/25232827 http://dx.doi.org/10.1371/journal.pone.0107566 Text en © 2014 Nassar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nassar, Farah J.
El Sabban, Maya
Zgheib, Nathalie K.
Tfayli, Arafat
Boulos, Fouad
Jabbour, Mark
Saghir, Nagi S. E. l.
Talhouk, Rabih
Bazarbachi, Ali
Calin, George A.
Nasr, Rihab
miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title_full miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title_fullStr miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title_full_unstemmed miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title_short miRNA as Potential Biomarkers of Breast Cancer in the Lebanese Population and in Young Women: A Pilot Study
title_sort mirna as potential biomarkers of breast cancer in the lebanese population and in young women: a pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169426/
https://www.ncbi.nlm.nih.gov/pubmed/25232827
http://dx.doi.org/10.1371/journal.pone.0107566
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