Cargando…
Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag
Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring protein dynamics in live cells that has provided many important biological insights. Although FRAP presumes that the conversion of a fluorophore from a bright to a dark state is irreversible, GFP as we...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169462/ https://www.ncbi.nlm.nih.gov/pubmed/25233348 http://dx.doi.org/10.1371/journal.pone.0107730 |