Semi-automated protocol for purification of Mycobacterium leprae from tissues using the gentleMACS™ Octo Dissociator

Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FPs). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very ef...

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Detalles Bibliográficos
Autores principales: Williams, Diana L., Adams, Linda B., Lahiri, Ramanuj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2014
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169666/
https://www.ncbi.nlm.nih.gov/pubmed/25019518
http://dx.doi.org/10.1016/j.mimet.2014.06.019
Descripción
Sumario:Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FPs). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.

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