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Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV
Bluetongue virus core protein VP6 is an ATP hydrolysis dependent RNA helicase. However, despite much study, the precise role of VP6 within the viral capsid and its structure remain unclear. To investigate the requirement of VP6 in BTV replication, we initiated a structural and biological study. Mult...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169673/ https://www.ncbi.nlm.nih.gov/pubmed/25128829 http://dx.doi.org/10.1016/j.bbrc.2014.08.028 |
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author | Matsuo, Eiko Leon, Esther Matthews, Steve J. Roy, Polly |
author_facet | Matsuo, Eiko Leon, Esther Matthews, Steve J. Roy, Polly |
author_sort | Matsuo, Eiko |
collection | PubMed |
description | Bluetongue virus core protein VP6 is an ATP hydrolysis dependent RNA helicase. However, despite much study, the precise role of VP6 within the viral capsid and its structure remain unclear. To investigate the requirement of VP6 in BTV replication, we initiated a structural and biological study. Multinuclear nuclear magnetic resonance spectra were assigned on his-tagged full-length VP6 (329 amino acid residues) as well as several truncated VP6 variants. The analysis revealed a large structured domain with two large loop regions that exhibit significant conformational exchange. One of the loops (amino acid position 34–130) could be removed without affecting the overall fold of the protein. Moreover, using a BTV reverse genetics system, it was possible to demonstrate that the VP6-truncated BTV was viable in BHK cells in the absence of any helper VP6 protein, suggesting that a large portion of this loop region is not absolutely required for BTV replication. |
format | Online Article Text |
id | pubmed-4169673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-41696732014-09-23 Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV Matsuo, Eiko Leon, Esther Matthews, Steve J. Roy, Polly Biochem Biophys Res Commun Article Bluetongue virus core protein VP6 is an ATP hydrolysis dependent RNA helicase. However, despite much study, the precise role of VP6 within the viral capsid and its structure remain unclear. To investigate the requirement of VP6 in BTV replication, we initiated a structural and biological study. Multinuclear nuclear magnetic resonance spectra were assigned on his-tagged full-length VP6 (329 amino acid residues) as well as several truncated VP6 variants. The analysis revealed a large structured domain with two large loop regions that exhibit significant conformational exchange. One of the loops (amino acid position 34–130) could be removed without affecting the overall fold of the protein. Moreover, using a BTV reverse genetics system, it was possible to demonstrate that the VP6-truncated BTV was viable in BHK cells in the absence of any helper VP6 protein, suggesting that a large portion of this loop region is not absolutely required for BTV replication. Elsevier 2014-09-05 /pmc/articles/PMC4169673/ /pubmed/25128829 http://dx.doi.org/10.1016/j.bbrc.2014.08.028 Text en © 2014 The Authors https://creativecommons.org/licenses/by/3.0/This work is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Article Matsuo, Eiko Leon, Esther Matthews, Steve J. Roy, Polly Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title | Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title_full | Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title_fullStr | Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title_full_unstemmed | Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title_short | Structure based modification of Bluetongue virus helicase protein VP6 to produce a viable VP6-truncated BTV |
title_sort | structure based modification of bluetongue virus helicase protein vp6 to produce a viable vp6-truncated btv |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169673/ https://www.ncbi.nlm.nih.gov/pubmed/25128829 http://dx.doi.org/10.1016/j.bbrc.2014.08.028 |
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