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Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin
Cytolethal distending toxin (CDT) produced by Campylobacter jejuni is a genotoxin that induces cell-cycle arrest and apoptosis in mammalian cells. Recent studies have demonstrated that prostate cancer (PCa) cells can acquire radio-resistance when DOC-2/DAB2 interactive protein (DAB2IP) is downregula...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170639/ https://www.ncbi.nlm.nih.gov/pubmed/25015118 |
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author | Lai, Chih-Ho Chang, Chia-Shuo Liu, Hsin-Ho Tsai, Yuh-Shyan Hsu, Feng-Ming Yu, Yung-Luen Lai, Cheng-Kuo Gandee, Leah Pong, Rey-Chen Hsu, Heng-Wei Yu, Lan Saha, Debabrata Hsieh, Jer-Tsong |
author_facet | Lai, Chih-Ho Chang, Chia-Shuo Liu, Hsin-Ho Tsai, Yuh-Shyan Hsu, Feng-Ming Yu, Yung-Luen Lai, Cheng-Kuo Gandee, Leah Pong, Rey-Chen Hsu, Heng-Wei Yu, Lan Saha, Debabrata Hsieh, Jer-Tsong |
author_sort | Lai, Chih-Ho |
collection | PubMed |
description | Cytolethal distending toxin (CDT) produced by Campylobacter jejuni is a genotoxin that induces cell-cycle arrest and apoptosis in mammalian cells. Recent studies have demonstrated that prostate cancer (PCa) cells can acquire radio-resistance when DOC-2/DAB2 interactive protein (DAB2IP) is downregulated. In this study, we showed that CDT could induce cell death in DAB2IP-deficient PCa cells. A combination of CDT and radiotherapy significantly elicited cell death in DAB2IP-deficient PCa cells by inhibiting the repair of ionizing radiation (IR)-induced DNA double-strand break (DSB) during G2/M arrest, which is triggered by ataxia telangiectasia mutated (ATM)-dependent DNA damage checkpoint responses. We also found that CDT administration significantly increased the efficacy of radiotherapy in a xenograft mouse model. These results indicate that CDT can be a potent therapeutic agent for radio-resistant PCa. |
format | Online Article Text |
id | pubmed-4170639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-41706392014-09-22 Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin Lai, Chih-Ho Chang, Chia-Shuo Liu, Hsin-Ho Tsai, Yuh-Shyan Hsu, Feng-Ming Yu, Yung-Luen Lai, Cheng-Kuo Gandee, Leah Pong, Rey-Chen Hsu, Heng-Wei Yu, Lan Saha, Debabrata Hsieh, Jer-Tsong Oncotarget Research Paper Cytolethal distending toxin (CDT) produced by Campylobacter jejuni is a genotoxin that induces cell-cycle arrest and apoptosis in mammalian cells. Recent studies have demonstrated that prostate cancer (PCa) cells can acquire radio-resistance when DOC-2/DAB2 interactive protein (DAB2IP) is downregulated. In this study, we showed that CDT could induce cell death in DAB2IP-deficient PCa cells. A combination of CDT and radiotherapy significantly elicited cell death in DAB2IP-deficient PCa cells by inhibiting the repair of ionizing radiation (IR)-induced DNA double-strand break (DSB) during G2/M arrest, which is triggered by ataxia telangiectasia mutated (ATM)-dependent DNA damage checkpoint responses. We also found that CDT administration significantly increased the efficacy of radiotherapy in a xenograft mouse model. These results indicate that CDT can be a potent therapeutic agent for radio-resistant PCa. Impact Journals LLC 2014-06-26 /pmc/articles/PMC4170639/ /pubmed/25015118 Text en Copyright: © 2014 Lai et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Lai, Chih-Ho Chang, Chia-Shuo Liu, Hsin-Ho Tsai, Yuh-Shyan Hsu, Feng-Ming Yu, Yung-Luen Lai, Cheng-Kuo Gandee, Leah Pong, Rey-Chen Hsu, Heng-Wei Yu, Lan Saha, Debabrata Hsieh, Jer-Tsong Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title | Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title_full | Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title_fullStr | Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title_full_unstemmed | Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title_short | Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
title_sort | sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170639/ https://www.ncbi.nlm.nih.gov/pubmed/25015118 |
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