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MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma
Therapeutic applications of microRNAs (miRNAs) in RAS-driven glioma were valuable, but their specific roles and functions have yet to be fully elucidated. Here, we firstly report that miR-143 directly targets the neuroblastoma RAS viral oncogene homolog (N-RAS) and functions as a tumor-suppressor in...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170647/ https://www.ncbi.nlm.nih.gov/pubmed/24980823 |
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author | Wang, Lin Shi, Zhu-mei Jiang, Cheng-fei Liu, Xue Chen, Qiu-dan Qian, Xu Li, Dong-mei Ge, Xin Wang, Xie-feng Liu, Ling-Zhi You, Yong-ping Liu, Ning Jiang, Bing-Hua |
author_facet | Wang, Lin Shi, Zhu-mei Jiang, Cheng-fei Liu, Xue Chen, Qiu-dan Qian, Xu Li, Dong-mei Ge, Xin Wang, Xie-feng Liu, Ling-Zhi You, Yong-ping Liu, Ning Jiang, Bing-Hua |
author_sort | Wang, Lin |
collection | PubMed |
description | Therapeutic applications of microRNAs (miRNAs) in RAS-driven glioma were valuable, but their specific roles and functions have yet to be fully elucidated. Here, we firstly report that miR-143 directly targets the neuroblastoma RAS viral oncogene homolog (N-RAS) and functions as a tumor-suppressor in glioma. Overexpression of miR-143 decreased the expression of N-RAS, inhibited PI3K/AKT, MAPK/ERK signaling, and attenuated the accumulation of p65 in nucleus of glioma cells. In human clinical specimens, miR-143 was downregulated where an adverse with N-RAS expression was observed. Furthermore, overexpression of miR-143 decreased glioma cell migration, invasion, tube formation and slowed tumor growth and angiogenesis in a manner associated with N-RAS downregulation in vitro and in vivo. Finally, miR-143 also sensitizes glioma cells to temozolomide (TMZ),the first-line drug for glioma treatment. Taken together, for the first time, our results demonstrate that miR-143 plays a significant role in inactivating the RAS signaling pathway through the inhibition of N-RAS, which may provide a novel therapeutic strategy for treatment of glioma and other RAS-driven cancers. |
format | Online Article Text |
id | pubmed-4170647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-41706472014-09-22 MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma Wang, Lin Shi, Zhu-mei Jiang, Cheng-fei Liu, Xue Chen, Qiu-dan Qian, Xu Li, Dong-mei Ge, Xin Wang, Xie-feng Liu, Ling-Zhi You, Yong-ping Liu, Ning Jiang, Bing-Hua Oncotarget Research Paper Therapeutic applications of microRNAs (miRNAs) in RAS-driven glioma were valuable, but their specific roles and functions have yet to be fully elucidated. Here, we firstly report that miR-143 directly targets the neuroblastoma RAS viral oncogene homolog (N-RAS) and functions as a tumor-suppressor in glioma. Overexpression of miR-143 decreased the expression of N-RAS, inhibited PI3K/AKT, MAPK/ERK signaling, and attenuated the accumulation of p65 in nucleus of glioma cells. In human clinical specimens, miR-143 was downregulated where an adverse with N-RAS expression was observed. Furthermore, overexpression of miR-143 decreased glioma cell migration, invasion, tube formation and slowed tumor growth and angiogenesis in a manner associated with N-RAS downregulation in vitro and in vivo. Finally, miR-143 also sensitizes glioma cells to temozolomide (TMZ),the first-line drug for glioma treatment. Taken together, for the first time, our results demonstrate that miR-143 plays a significant role in inactivating the RAS signaling pathway through the inhibition of N-RAS, which may provide a novel therapeutic strategy for treatment of glioma and other RAS-driven cancers. Impact Journals LLC 2014-06-18 /pmc/articles/PMC4170647/ /pubmed/24980823 Text en Copyright: © 2014 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Lin Shi, Zhu-mei Jiang, Cheng-fei Liu, Xue Chen, Qiu-dan Qian, Xu Li, Dong-mei Ge, Xin Wang, Xie-feng Liu, Ling-Zhi You, Yong-ping Liu, Ning Jiang, Bing-Hua MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title | MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title_full | MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title_fullStr | MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title_full_unstemmed | MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title_short | MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma |
title_sort | mir-143 acts as a tumor suppressor by targeting n-ras and enhances temozolomide-induced apoptosis in glioma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170647/ https://www.ncbi.nlm.nih.gov/pubmed/24980823 |
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