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Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNA

BACKGROUND: Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in librari...

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Detalles Bibliográficos
Autores principales:	Yadav, Rajiv Kumar, Barbi, Florian, Ziller, Antoine, Luis, Patricia, Marmeisse, Roland, Reddy, M Sudhakara, Fraissinet-Tachet, Laurence
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170940/ https://www.ncbi.nlm.nih.gov/pubmed/25183040 http://dx.doi.org/10.1186/1472-6750-14-80

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