Cargando…

Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3

In this study, we isolated an endophytic quinclorac-degrading bacterium strain Q3 from the root of tobacco grown in quinclorac contaminated soil. Based on morphological characteristics, Biolog identification, and 16S rDNA sequence analysis, we identified strain Q3 as Bacillus megaterium. We investig...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Min, Luo, Kun, Wang, Yunsheng, Zeng, Aiping, Zhou, Xiaomao, Luo, Feng, Bai, Lianyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171507/
https://www.ncbi.nlm.nih.gov/pubmed/25244184
http://dx.doi.org/10.1371/journal.pone.0108012
_version_ 1782335897592659968
author Liu, Min
Luo, Kun
Wang, Yunsheng
Zeng, Aiping
Zhou, Xiaomao
Luo, Feng
Bai, Lianyang
author_facet Liu, Min
Luo, Kun
Wang, Yunsheng
Zeng, Aiping
Zhou, Xiaomao
Luo, Feng
Bai, Lianyang
author_sort Liu, Min
collection PubMed
description In this study, we isolated an endophytic quinclorac-degrading bacterium strain Q3 from the root of tobacco grown in quinclorac contaminated soil. Based on morphological characteristics, Biolog identification, and 16S rDNA sequence analysis, we identified strain Q3 as Bacillus megaterium. We investigated the effects of temperature, pH, inoculation size, and initial quinclorac concentration on growth and degrading efficiency of Q3. Under the optimal degrading condition, Q3 could degrade 93% of quinclorac from the initial concentration of 20 mg/L in seven days. We analyzed the degradation products of quinclorac using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The major degradation products by Q3 were different from those of previously identified quinclorac degrading strains, which suggests that Q3 may employ new pathways for quinclorac degradation. Our indoor pot experiments demonstrated that Q3 can effectively alleviate the quinclorac phytotoxicity in tobacco. As the first endophytic microbial that is capable of degrading quinclorac, Q3 can be a good bioremediation bacterium for quinclorac phytotoxicity.
format Online
Article
Text
id pubmed-4171507
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41715072014-09-25 Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3 Liu, Min Luo, Kun Wang, Yunsheng Zeng, Aiping Zhou, Xiaomao Luo, Feng Bai, Lianyang PLoS One Research Article In this study, we isolated an endophytic quinclorac-degrading bacterium strain Q3 from the root of tobacco grown in quinclorac contaminated soil. Based on morphological characteristics, Biolog identification, and 16S rDNA sequence analysis, we identified strain Q3 as Bacillus megaterium. We investigated the effects of temperature, pH, inoculation size, and initial quinclorac concentration on growth and degrading efficiency of Q3. Under the optimal degrading condition, Q3 could degrade 93% of quinclorac from the initial concentration of 20 mg/L in seven days. We analyzed the degradation products of quinclorac using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The major degradation products by Q3 were different from those of previously identified quinclorac degrading strains, which suggests that Q3 may employ new pathways for quinclorac degradation. Our indoor pot experiments demonstrated that Q3 can effectively alleviate the quinclorac phytotoxicity in tobacco. As the first endophytic microbial that is capable of degrading quinclorac, Q3 can be a good bioremediation bacterium for quinclorac phytotoxicity. Public Library of Science 2014-09-22 /pmc/articles/PMC4171507/ /pubmed/25244184 http://dx.doi.org/10.1371/journal.pone.0108012 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Min
Luo, Kun
Wang, Yunsheng
Zeng, Aiping
Zhou, Xiaomao
Luo, Feng
Bai, Lianyang
Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title_full Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title_fullStr Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title_full_unstemmed Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title_short Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3
title_sort isolation, identification and characteristics of an endophytic quinclorac degrading bacterium bacillus megaterium q3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171507/
https://www.ncbi.nlm.nih.gov/pubmed/25244184
http://dx.doi.org/10.1371/journal.pone.0108012
work_keys_str_mv AT liumin isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT luokun isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT wangyunsheng isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT zengaiping isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT zhouxiaomao isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT luofeng isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3
AT bailianyang isolationidentificationandcharacteristicsofanendophyticquincloracdegradingbacteriumbacillusmegateriumq3