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Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm
BACKGROUND: Abdominal aortic aneurysm (AAA) is a life-threatening disease and its prevalence rate increases with social aging. The degradation of elastic is an important factor in the formation of AAA. METHODS: Adipose derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) were is...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171524/ https://www.ncbi.nlm.nih.gov/pubmed/25243605 http://dx.doi.org/10.1371/journal.pone.0108105 |
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author | Tian, Xiaohong Fan, Jun Yu, Miao Zhao, Yu Fang, Yan Bai, Shuling Hou, Weijian Tong, Hao |
author_facet | Tian, Xiaohong Fan, Jun Yu, Miao Zhao, Yu Fang, Yan Bai, Shuling Hou, Weijian Tong, Hao |
author_sort | Tian, Xiaohong |
collection | PubMed |
description | BACKGROUND: Abdominal aortic aneurysm (AAA) is a life-threatening disease and its prevalence rate increases with social aging. The degradation of elastic is an important factor in the formation of AAA. METHODS: Adipose derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) were isolated from rats, and identified by Oil red O and alizarin red staining after adipogenesis and osteogenesis induction. In addition, ADSCs were also identified by flow cytometry with CD markers. AAA model in rats was established, and smooth muscle cells (SMCs) were isolated from AAA aortic wall and identified by immunohistochemistry. ADSCs or BMSCs were co-cultured with AAA aortic wall for in vitro experiment, and ADSCs were injected into AAA model for in vivo test. Then orcein staining was used for observing the morphology of elastic fiber, Western blot and real-time PCR were used respectively to detect the protein and gene expression of elastin, gelatinases spectrum analysis was used to detect the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9. RESULTS: Lots of red lipid droplets were visible by Oil red O staining after adipogenesis induction, and black calcium nodules appeared by alizarin red staining after osteogenesis induction. The results of flow cytometry showed that ADSCs expressed CD44 and CD105, but exhibited negligible expression of CD31 and CD45. SMCs exhibited spindle-like morphology and α-actin protein was positive in cytoplasm. After co-cultured with ADSCs or BMSCs, the elastic fiber recovered normal winding shape, both the gene and protein expression of elastin increased, and the activity of MMP-2 decreased. The in vivo result was similar to that of in vitro. CONCLUSIONS: ADSCs promote the expression of elastin in SMCs and contribute to the reconstruction of elastic fiber, which may provide new ideas for treating AAA. |
format | Online Article Text |
id | pubmed-4171524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41715242014-09-25 Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm Tian, Xiaohong Fan, Jun Yu, Miao Zhao, Yu Fang, Yan Bai, Shuling Hou, Weijian Tong, Hao PLoS One Research Article BACKGROUND: Abdominal aortic aneurysm (AAA) is a life-threatening disease and its prevalence rate increases with social aging. The degradation of elastic is an important factor in the formation of AAA. METHODS: Adipose derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) were isolated from rats, and identified by Oil red O and alizarin red staining after adipogenesis and osteogenesis induction. In addition, ADSCs were also identified by flow cytometry with CD markers. AAA model in rats was established, and smooth muscle cells (SMCs) were isolated from AAA aortic wall and identified by immunohistochemistry. ADSCs or BMSCs were co-cultured with AAA aortic wall for in vitro experiment, and ADSCs were injected into AAA model for in vivo test. Then orcein staining was used for observing the morphology of elastic fiber, Western blot and real-time PCR were used respectively to detect the protein and gene expression of elastin, gelatinases spectrum analysis was used to detect the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9. RESULTS: Lots of red lipid droplets were visible by Oil red O staining after adipogenesis induction, and black calcium nodules appeared by alizarin red staining after osteogenesis induction. The results of flow cytometry showed that ADSCs expressed CD44 and CD105, but exhibited negligible expression of CD31 and CD45. SMCs exhibited spindle-like morphology and α-actin protein was positive in cytoplasm. After co-cultured with ADSCs or BMSCs, the elastic fiber recovered normal winding shape, both the gene and protein expression of elastin increased, and the activity of MMP-2 decreased. The in vivo result was similar to that of in vitro. CONCLUSIONS: ADSCs promote the expression of elastin in SMCs and contribute to the reconstruction of elastic fiber, which may provide new ideas for treating AAA. Public Library of Science 2014-09-22 /pmc/articles/PMC4171524/ /pubmed/25243605 http://dx.doi.org/10.1371/journal.pone.0108105 Text en © 2014 Tian et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tian, Xiaohong Fan, Jun Yu, Miao Zhao, Yu Fang, Yan Bai, Shuling Hou, Weijian Tong, Hao Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title | Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title_full | Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title_fullStr | Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title_full_unstemmed | Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title_short | Adipose Stem Cells Promote Smooth Muscle Cells to Secrete Elastin in Rat Abdominal Aortic Aneurysm |
title_sort | adipose stem cells promote smooth muscle cells to secrete elastin in rat abdominal aortic aneurysm |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171524/ https://www.ncbi.nlm.nih.gov/pubmed/25243605 http://dx.doi.org/10.1371/journal.pone.0108105 |
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