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Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget

The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-po...

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Autores principales: Hamada, Taiji, Souda, Masakazu, Yoshimura, Takuya, Sasaguri, Shoko, Hatanaka, Kazuhito, Tasaki, Takashi, Yoshioka, Takako, Ohi, Yasuyo, Yamada, Sohsuke, Tsutsui, Masato, Umekita, Yoshihisa, Tanimoto, Akihide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171614/
https://www.ncbi.nlm.nih.gov/pubmed/25153723
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author Hamada, Taiji
Souda, Masakazu
Yoshimura, Takuya
Sasaguri, Shoko
Hatanaka, Kazuhito
Tasaki, Takashi
Yoshioka, Takako
Ohi, Yasuyo
Yamada, Sohsuke
Tsutsui, Masato
Umekita, Yoshihisa
Tanimoto, Akihide
author_facet Hamada, Taiji
Souda, Masakazu
Yoshimura, Takuya
Sasaguri, Shoko
Hatanaka, Kazuhito
Tasaki, Takashi
Yoshioka, Takako
Ohi, Yasuyo
Yamada, Sohsuke
Tsutsui, Masato
Umekita, Yoshihisa
Tanimoto, Akihide
author_sort Hamada, Taiji
collection PubMed
description The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-positive MCF-7 and ER-negative SK-BR-3 cells expressed PCP4/PEP19. In the MCF-7 cells, cell proliferation was estrogen-dependent, and PCP4/PEP19 expression was induced by estrogen. In both cell lines, PCP4/PEP19 knockdown induced apoptosis and slightly decreased Akt phosphorylation. Knockdown of calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1), resulting in decreased phospho-Akt(Thr308), enhanced apoptosis in SK-BR-3 but not in MCF-7 cells. CaMKK2 knockdown moderately decreased phospho-Akt(Thr308) and increased apoptosis in MCF-7 cells but not in SK-BR-3 cells. These data indicated that PCP4/PEP19 regulates apoptosis but exact mechanism is still unknown. PCP4/PEP19 can therefore potentially serve as independent oncotarget for therapy of PCP4/PEP19-positive breast cancers irrespective of ER expression.
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spelling pubmed-41716142014-09-23 Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget Hamada, Taiji Souda, Masakazu Yoshimura, Takuya Sasaguri, Shoko Hatanaka, Kazuhito Tasaki, Takashi Yoshioka, Takako Ohi, Yasuyo Yamada, Sohsuke Tsutsui, Masato Umekita, Yoshihisa Tanimoto, Akihide Oncotarget Research Paper The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-positive MCF-7 and ER-negative SK-BR-3 cells expressed PCP4/PEP19. In the MCF-7 cells, cell proliferation was estrogen-dependent, and PCP4/PEP19 expression was induced by estrogen. In both cell lines, PCP4/PEP19 knockdown induced apoptosis and slightly decreased Akt phosphorylation. Knockdown of calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1), resulting in decreased phospho-Akt(Thr308), enhanced apoptosis in SK-BR-3 but not in MCF-7 cells. CaMKK2 knockdown moderately decreased phospho-Akt(Thr308) and increased apoptosis in MCF-7 cells but not in SK-BR-3 cells. These data indicated that PCP4/PEP19 regulates apoptosis but exact mechanism is still unknown. PCP4/PEP19 can therefore potentially serve as independent oncotarget for therapy of PCP4/PEP19-positive breast cancers irrespective of ER expression. Impact Journals LLC 2014-07-08 /pmc/articles/PMC4171614/ /pubmed/25153723 Text en Copyright: © 2014 Hamada et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Hamada, Taiji
Souda, Masakazu
Yoshimura, Takuya
Sasaguri, Shoko
Hatanaka, Kazuhito
Tasaki, Takashi
Yoshioka, Takako
Ohi, Yasuyo
Yamada, Sohsuke
Tsutsui, Masato
Umekita, Yoshihisa
Tanimoto, Akihide
Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title_full Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title_fullStr Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title_full_unstemmed Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title_short Anti-apoptotic Effects of PCP4/PEP19 in Human Breast Cancer Cell Lines: A Novel Oncotarget
title_sort anti-apoptotic effects of pcp4/pep19 in human breast cancer cell lines: a novel oncotarget
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171614/
https://www.ncbi.nlm.nih.gov/pubmed/25153723
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