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Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
BACKGROUND: An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171716/ https://www.ncbi.nlm.nih.gov/pubmed/25250056 http://dx.doi.org/10.1186/1746-4811-10-29 |