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Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

BACKGROUND: An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These...

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Detalles Bibliográficos
Autores principales:	Athman, Asmini, Tanz, Sandra K, Conn, Vanessa M, Jordans, Charlotte, Mayo, Gwenda M, Ng, Weng W, Burton, Rachel A, Conn, Simon J, Gilliham, Matthew
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171716/ https://www.ncbi.nlm.nih.gov/pubmed/25250056 http://dx.doi.org/10.1186/1746-4811-10-29

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