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Intestinal anastomotic injury alters spatially defined microbiome composition and function

BACKGROUND: When diseased intestine (i.e., from colon cancer, diverticulitis) requires resection, its reconnection (termed anastomosis) can be complicated by non-healing of the newly joined intestine resulting in spillage of intestinal contents into the abdominal cavity (termed anastomotic leakage)....

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Autores principales: Shogan, Benjamin D, Smith, Daniel P, Christley, Scott, Gilbert, Jack A, Zaborina, Olga, Alverdy, John C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171717/
https://www.ncbi.nlm.nih.gov/pubmed/25250176
http://dx.doi.org/10.1186/2049-2618-2-35
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author Shogan, Benjamin D
Smith, Daniel P
Christley, Scott
Gilbert, Jack A
Zaborina, Olga
Alverdy, John C
author_facet Shogan, Benjamin D
Smith, Daniel P
Christley, Scott
Gilbert, Jack A
Zaborina, Olga
Alverdy, John C
author_sort Shogan, Benjamin D
collection PubMed
description BACKGROUND: When diseased intestine (i.e., from colon cancer, diverticulitis) requires resection, its reconnection (termed anastomosis) can be complicated by non-healing of the newly joined intestine resulting in spillage of intestinal contents into the abdominal cavity (termed anastomotic leakage). While it is suspected that the intestinal microbiota have the capacity to both accelerate and complicate anastomotic healing, the associated genotypes and functions have not been characterized. RESULTS: Using 16S rRNA amplicon sequencing of samples collected on the day of surgery (postoperative day 0 (POD0)) and the 6th day following surgery (postoperative day 0 (POD6)), we analyzed the changes in luminal versus tissue-associated microbiota at anastomotic sites created in the colon of rats. Results indicated that anastomotic injury induced significant changes in the anastomotic tissue-associated microbiota with minimal differences in the luminal microbiota. The most striking difference was a 500-fold and 200-fold increase in the relative abundance of Enterococcus and Escherichia/Shigella, respectively. Functional profiling predicted the predominance of bacterial virulence-associated pathways in post-anastomotic tissues, including production of hemolysin, cytolethal toxins, fimbriae, invasins, cytotoxic necrotizing factors, and coccolysin. CONCLUSION: Taken together, our results suggest that compositional and functional changes accompany anastomotic tissues and may potentially accelerate or complicate anastomotic healing.
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spelling pubmed-41717172014-09-24 Intestinal anastomotic injury alters spatially defined microbiome composition and function Shogan, Benjamin D Smith, Daniel P Christley, Scott Gilbert, Jack A Zaborina, Olga Alverdy, John C Microbiome Research BACKGROUND: When diseased intestine (i.e., from colon cancer, diverticulitis) requires resection, its reconnection (termed anastomosis) can be complicated by non-healing of the newly joined intestine resulting in spillage of intestinal contents into the abdominal cavity (termed anastomotic leakage). While it is suspected that the intestinal microbiota have the capacity to both accelerate and complicate anastomotic healing, the associated genotypes and functions have not been characterized. RESULTS: Using 16S rRNA amplicon sequencing of samples collected on the day of surgery (postoperative day 0 (POD0)) and the 6th day following surgery (postoperative day 0 (POD6)), we analyzed the changes in luminal versus tissue-associated microbiota at anastomotic sites created in the colon of rats. Results indicated that anastomotic injury induced significant changes in the anastomotic tissue-associated microbiota with minimal differences in the luminal microbiota. The most striking difference was a 500-fold and 200-fold increase in the relative abundance of Enterococcus and Escherichia/Shigella, respectively. Functional profiling predicted the predominance of bacterial virulence-associated pathways in post-anastomotic tissues, including production of hemolysin, cytolethal toxins, fimbriae, invasins, cytotoxic necrotizing factors, and coccolysin. CONCLUSION: Taken together, our results suggest that compositional and functional changes accompany anastomotic tissues and may potentially accelerate or complicate anastomotic healing. BioMed Central 2014-09-15 /pmc/articles/PMC4171717/ /pubmed/25250176 http://dx.doi.org/10.1186/2049-2618-2-35 Text en Copyright © 2014 Shogan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Shogan, Benjamin D
Smith, Daniel P
Christley, Scott
Gilbert, Jack A
Zaborina, Olga
Alverdy, John C
Intestinal anastomotic injury alters spatially defined microbiome composition and function
title Intestinal anastomotic injury alters spatially defined microbiome composition and function
title_full Intestinal anastomotic injury alters spatially defined microbiome composition and function
title_fullStr Intestinal anastomotic injury alters spatially defined microbiome composition and function
title_full_unstemmed Intestinal anastomotic injury alters spatially defined microbiome composition and function
title_short Intestinal anastomotic injury alters spatially defined microbiome composition and function
title_sort intestinal anastomotic injury alters spatially defined microbiome composition and function
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171717/
https://www.ncbi.nlm.nih.gov/pubmed/25250176
http://dx.doi.org/10.1186/2049-2618-2-35
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