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Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene exp...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171766/ https://www.ncbi.nlm.nih.gov/pubmed/25249769 |
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author | Del Valle, Paulo Roberto Milani, Cintia Brentani, Maria Mitzi Katayama, Maria Lucia Hirata de Lyra, Eduardo Carneiro Carraro, Dirce Maria Brentani, Helena Puga, Renato Lima, Leandro A. Rozenchan, Patricia Bortman Nunes, Bárbara dos Santos Góes, João Carlos Guedes Sampaio Azevedo Koike Folgueira, Maria Aparecida |
author_facet | Del Valle, Paulo Roberto Milani, Cintia Brentani, Maria Mitzi Katayama, Maria Lucia Hirata de Lyra, Eduardo Carneiro Carraro, Dirce Maria Brentani, Helena Puga, Renato Lima, Leandro A. Rozenchan, Patricia Bortman Nunes, Bárbara dos Santos Góes, João Carlos Guedes Sampaio Azevedo Koike Folgueira, Maria Aparecida |
author_sort | Del Valle, Paulo Roberto |
collection | PubMed |
description | Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs. |
format | Online Article Text |
id | pubmed-4171766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-41717662014-09-23 Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients Del Valle, Paulo Roberto Milani, Cintia Brentani, Maria Mitzi Katayama, Maria Lucia Hirata de Lyra, Eduardo Carneiro Carraro, Dirce Maria Brentani, Helena Puga, Renato Lima, Leandro A. Rozenchan, Patricia Bortman Nunes, Bárbara dos Santos Góes, João Carlos Guedes Sampaio Azevedo Koike Folgueira, Maria Aparecida Genet Mol Biol Human and Medical Genetics Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs. Sociedade Brasileira de Genética 2014-09 /pmc/articles/PMC4171766/ /pubmed/25249769 Text en Copyright © 2014, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Human and Medical Genetics Del Valle, Paulo Roberto Milani, Cintia Brentani, Maria Mitzi Katayama, Maria Lucia Hirata de Lyra, Eduardo Carneiro Carraro, Dirce Maria Brentani, Helena Puga, Renato Lima, Leandro A. Rozenchan, Patricia Bortman Nunes, Bárbara dos Santos Góes, João Carlos Guedes Sampaio Azevedo Koike Folgueira, Maria Aparecida Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title | Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_full | Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_fullStr | Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_full_unstemmed | Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_short | Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
title_sort | transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients |
topic | Human and Medical Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171766/ https://www.ncbi.nlm.nih.gov/pubmed/25249769 |
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