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An expanded tool kit for the auxin-inducible degron system in budding yeast

Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a seri...

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Detalles Bibliográficos
Autores principales: Morawska, Magdalena, Ulrich, Helle D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171812/
https://www.ncbi.nlm.nih.gov/pubmed/23836714
http://dx.doi.org/10.1002/yea.2967
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author Morawska, Magdalena
Ulrich, Helle D
author_facet Morawska, Magdalena
Ulrich, Helle D
author_sort Morawska, Magdalena
collection PubMed
description Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR-based genomic tagging strategies, allow for C- or N-terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd.
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spelling pubmed-41718122014-10-08 An expanded tool kit for the auxin-inducible degron system in budding yeast Morawska, Magdalena Ulrich, Helle D Yeast Research Articles Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR-based genomic tagging strategies, allow for C- or N-terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd. Blackwell Publishing Ltd 2013-09 2013-07-23 /pmc/articles/PMC4171812/ /pubmed/23836714 http://dx.doi.org/10.1002/yea.2967 Text en Copyright © 2013 John Wiley & Sons, Ltd. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Morawska, Magdalena
Ulrich, Helle D
An expanded tool kit for the auxin-inducible degron system in budding yeast
title An expanded tool kit for the auxin-inducible degron system in budding yeast
title_full An expanded tool kit for the auxin-inducible degron system in budding yeast
title_fullStr An expanded tool kit for the auxin-inducible degron system in budding yeast
title_full_unstemmed An expanded tool kit for the auxin-inducible degron system in budding yeast
title_short An expanded tool kit for the auxin-inducible degron system in budding yeast
title_sort expanded tool kit for the auxin-inducible degron system in budding yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171812/
https://www.ncbi.nlm.nih.gov/pubmed/23836714
http://dx.doi.org/10.1002/yea.2967
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