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DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL

Sandflies transmit pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern Brazil. Sandflies were collected with Falcão and Shannon traps. After dissection in search of flagellates in digestive tubes a...

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Autores principales: Neitzke-Abreu, Herintha Coeto, Reinhold-Castro, Kárin Rosi, Venazzi, Mateus Sabaini, Scodro, Regiane Bertin de Lima, Dias, Alessandra de Cassia, Silveira, Thaís Gomes Verzignassi, Teodoro, Ueslei, Lonardoni, Maria Valdrinez Campana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172109/
https://www.ncbi.nlm.nih.gov/pubmed/25229218
http://dx.doi.org/10.1590/S0036-46652014000500004
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author Neitzke-Abreu, Herintha Coeto
Reinhold-Castro, Kárin Rosi
Venazzi, Mateus Sabaini
Scodro, Regiane Bertin de Lima
Dias, Alessandra de Cassia
Silveira, Thaís Gomes Verzignassi
Teodoro, Ueslei
Lonardoni, Maria Valdrinez Campana
author_facet Neitzke-Abreu, Herintha Coeto
Reinhold-Castro, Kárin Rosi
Venazzi, Mateus Sabaini
Scodro, Regiane Bertin de Lima
Dias, Alessandra de Cassia
Silveira, Thaís Gomes Verzignassi
Teodoro, Ueslei
Lonardoni, Maria Valdrinez Campana
author_sort Neitzke-Abreu, Herintha Coeto
collection PubMed
description Sandflies transmit pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern Brazil. Sandflies were collected with Falcão and Shannon traps. After dissection in search of flagellates in digestive tubes and identification of the species, female sandflies were submitted to the Multiplex Polymerase Chain Reaction (multiplex PCR) for detection of the fragment of the kDNA of Leishmania (Viannia) and the fragment from the IVS6 cacophony gene region of the phlebotomine insects. The analysis was performed in pools containing seven to 12 guts from females of the same species. A total of 510 female sandflies were analyzed, including nine Migonemyia migonei, 17 Pintomyia fischeri, 216 Nyssomyia neivai, and 268 Nyssomyia whitmani. Although none of the females was found naturally infected by flagellates through dissection, the fragment of DNA from Leishmania (Viannia) was shown by multiplex PCR in one sample of Ny. neivai (0.46%) and three samples of Ny. whitmani (1.12%). It was concluded that Ny. neivai and Ny. whitmani are susceptible to Leishmania infection, and that multiplex PCR can be used in epidemiological studies to detect the natural infection of the sandfly vector, because of its sensitivity, specificity and feasibility.
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spelling pubmed-41721092014-10-08 DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL Neitzke-Abreu, Herintha Coeto Reinhold-Castro, Kárin Rosi Venazzi, Mateus Sabaini Scodro, Regiane Bertin de Lima Dias, Alessandra de Cassia Silveira, Thaís Gomes Verzignassi Teodoro, Ueslei Lonardoni, Maria Valdrinez Campana Rev Inst Med Trop Sao Paulo Leishmaniasis Sandflies transmit pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern Brazil. Sandflies were collected with Falcão and Shannon traps. After dissection in search of flagellates in digestive tubes and identification of the species, female sandflies were submitted to the Multiplex Polymerase Chain Reaction (multiplex PCR) for detection of the fragment of the kDNA of Leishmania (Viannia) and the fragment from the IVS6 cacophony gene region of the phlebotomine insects. The analysis was performed in pools containing seven to 12 guts from females of the same species. A total of 510 female sandflies were analyzed, including nine Migonemyia migonei, 17 Pintomyia fischeri, 216 Nyssomyia neivai, and 268 Nyssomyia whitmani. Although none of the females was found naturally infected by flagellates through dissection, the fragment of DNA from Leishmania (Viannia) was shown by multiplex PCR in one sample of Ny. neivai (0.46%) and three samples of Ny. whitmani (1.12%). It was concluded that Ny. neivai and Ny. whitmani are susceptible to Leishmania infection, and that multiplex PCR can be used in epidemiological studies to detect the natural infection of the sandfly vector, because of its sensitivity, specificity and feasibility. Instituto de Medicina Tropical 2014 /pmc/articles/PMC4172109/ /pubmed/25229218 http://dx.doi.org/10.1590/S0036-46652014000500004 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Leishmaniasis
Neitzke-Abreu, Herintha Coeto
Reinhold-Castro, Kárin Rosi
Venazzi, Mateus Sabaini
Scodro, Regiane Bertin de Lima
Dias, Alessandra de Cassia
Silveira, Thaís Gomes Verzignassi
Teodoro, Ueslei
Lonardoni, Maria Valdrinez Campana
DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title_full DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title_fullStr DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title_full_unstemmed DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title_short DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL
title_sort detection of leishmania (viannia) in nyssomyia neivai and nyssomyia whitmani by multiplex polymerase chain reaction, in southern brazil
topic Leishmaniasis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172109/
https://www.ncbi.nlm.nih.gov/pubmed/25229218
http://dx.doi.org/10.1590/S0036-46652014000500004
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