Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

[Image: see text] Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach t...

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Autores principales: Sundermeier, Thomas R., Jin, Hui, Kleinjan, Matthew L., Mustafi, Debarshi, Licatalosi, Donny D., Palczewski, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172207/
https://www.ncbi.nlm.nih.gov/pubmed/25204418
http://dx.doi.org/10.1021/bi500966b
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author Sundermeier, Thomas R.
Jin, Hui
Kleinjan, Matthew L.
Mustafi, Debarshi
Licatalosi, Donny D.
Palczewski, Krzysztof
author_facet Sundermeier, Thomas R.
Jin, Hui
Kleinjan, Matthew L.
Mustafi, Debarshi
Licatalosi, Donny D.
Palczewski, Krzysztof
author_sort Sundermeier, Thomas R.
collection PubMed
description [Image: see text] Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 high-confidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases.
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spelling pubmed-41722072015-09-10 Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina Sundermeier, Thomas R. Jin, Hui Kleinjan, Matthew L. Mustafi, Debarshi Licatalosi, Donny D. Palczewski, Krzysztof Biochemistry [Image: see text] Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 high-confidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases. American Chemical Society 2014-09-10 2014-09-23 /pmc/articles/PMC4172207/ /pubmed/25204418 http://dx.doi.org/10.1021/bi500966b Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Sundermeier, Thomas R.
Jin, Hui
Kleinjan, Matthew L.
Mustafi, Debarshi
Licatalosi, Donny D.
Palczewski, Krzysztof
Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title_full Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title_fullStr Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title_full_unstemmed Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title_short Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina
title_sort argonaute high-throughput sequencing of rnas isolated by cross-linking immunoprecipitation reveals a snapshot of mirna gene regulation in the mammalian retina
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172207/
https://www.ncbi.nlm.nih.gov/pubmed/25204418
http://dx.doi.org/10.1021/bi500966b
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