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Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays

[Image: see text] We describe a manufacturable and scalable method for fabrication of multiscale wrinkled silica (SiO(2)) structures on shrink-wrap film to enhance fluorescence signals in DNA fluorescence microarrays. We are able to enhance the fluorescence signal of hybridized DNA by more than 120...

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Autores principales: Sharma, Himanshu, Wood, Jennifer B., Lin, Sophia, Corn, Robert M., Khine, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172299/
https://www.ncbi.nlm.nih.gov/pubmed/25191785
http://dx.doi.org/10.1021/la501123b
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author Sharma, Himanshu
Wood, Jennifer B.
Lin, Sophia
Corn, Robert M.
Khine, Michelle
author_facet Sharma, Himanshu
Wood, Jennifer B.
Lin, Sophia
Corn, Robert M.
Khine, Michelle
author_sort Sharma, Himanshu
collection PubMed
description [Image: see text] We describe a manufacturable and scalable method for fabrication of multiscale wrinkled silica (SiO(2)) structures on shrink-wrap film to enhance fluorescence signals in DNA fluorescence microarrays. We are able to enhance the fluorescence signal of hybridized DNA by more than 120 fold relative to a planar glass slide. Notably, our substrate has improved detection sensitivity (280 pM) relative to planar glass slide (11 nM). Furthermore, this is accompanied by a 30–45 times improvement in the signal-to-noise ratio (SNR). Unlike metal enhanced fluorescence (MEF) based enhancements, this is a far-field and uniform effect based on surface concentration and photophysical effects from the nano- to microscale SiO(2) structures. Notably, the photophysical effects contribute an almost 2.5 fold enhancement over the concentration effects alone. Therefore, this simple and robust method offers an efficient technique to enhance the detection capabilities of fluorescence based DNA microarrays.
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spelling pubmed-41722992015-09-05 Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays Sharma, Himanshu Wood, Jennifer B. Lin, Sophia Corn, Robert M. Khine, Michelle Langmuir [Image: see text] We describe a manufacturable and scalable method for fabrication of multiscale wrinkled silica (SiO(2)) structures on shrink-wrap film to enhance fluorescence signals in DNA fluorescence microarrays. We are able to enhance the fluorescence signal of hybridized DNA by more than 120 fold relative to a planar glass slide. Notably, our substrate has improved detection sensitivity (280 pM) relative to planar glass slide (11 nM). Furthermore, this is accompanied by a 30–45 times improvement in the signal-to-noise ratio (SNR). Unlike metal enhanced fluorescence (MEF) based enhancements, this is a far-field and uniform effect based on surface concentration and photophysical effects from the nano- to microscale SiO(2) structures. Notably, the photophysical effects contribute an almost 2.5 fold enhancement over the concentration effects alone. Therefore, this simple and robust method offers an efficient technique to enhance the detection capabilities of fluorescence based DNA microarrays. American Chemical Society 2014-09-05 2014-09-23 /pmc/articles/PMC4172299/ /pubmed/25191785 http://dx.doi.org/10.1021/la501123b Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Sharma, Himanshu
Wood, Jennifer B.
Lin, Sophia
Corn, Robert M.
Khine, Michelle
Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title_full Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title_fullStr Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title_full_unstemmed Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title_short Shrink-Induced Silica Multiscale Structures for Enhanced Fluorescence from DNA Microarrays
title_sort shrink-induced silica multiscale structures for enhanced fluorescence from dna microarrays
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172299/
https://www.ncbi.nlm.nih.gov/pubmed/25191785
http://dx.doi.org/10.1021/la501123b
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