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A genomics approach identifies senescence-specific gene expression regulation
Replicative senescence is a fundamental tumor-suppressive mechanism triggered by telomere erosion that results in a permanent cell cycle arrest. To understand the impact of telomere shortening on gene expression, we analyzed the transcriptome of diploid human fibroblasts as they progressed toward an...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172521/ https://www.ncbi.nlm.nih.gov/pubmed/24863242 http://dx.doi.org/10.1111/acel.12234 |
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author | Lackner, Daniel H Hayashi, Makoto T Cesare, Anthony J Karlseder, Jan |
author_facet | Lackner, Daniel H Hayashi, Makoto T Cesare, Anthony J Karlseder, Jan |
author_sort | Lackner, Daniel H |
collection | PubMed |
description | Replicative senescence is a fundamental tumor-suppressive mechanism triggered by telomere erosion that results in a permanent cell cycle arrest. To understand the impact of telomere shortening on gene expression, we analyzed the transcriptome of diploid human fibroblasts as they progressed toward and entered into senescence. We distinguished novel transcription regulation due to replicative senescence by comparing senescence-specific expression profiles to profiles from cells arrested by DNA damage or serum starvation. Only a small specific subset of genes was identified that was truly senescence-regulated and changes in gene expression were exacerbated from presenescent to senescent cells. The majority of gene expression regulation in replicative senescence was shown to occur due to telomere shortening, as exogenous telomerase activity reverted most of these changes. |
format | Online Article Text |
id | pubmed-4172521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41725212015-02-19 A genomics approach identifies senescence-specific gene expression regulation Lackner, Daniel H Hayashi, Makoto T Cesare, Anthony J Karlseder, Jan Aging Cell Short Takes Replicative senescence is a fundamental tumor-suppressive mechanism triggered by telomere erosion that results in a permanent cell cycle arrest. To understand the impact of telomere shortening on gene expression, we analyzed the transcriptome of diploid human fibroblasts as they progressed toward and entered into senescence. We distinguished novel transcription regulation due to replicative senescence by comparing senescence-specific expression profiles to profiles from cells arrested by DNA damage or serum starvation. Only a small specific subset of genes was identified that was truly senescence-regulated and changes in gene expression were exacerbated from presenescent to senescent cells. The majority of gene expression regulation in replicative senescence was shown to occur due to telomere shortening, as exogenous telomerase activity reverted most of these changes. BlackWell Publishing Ltd 2014-10 2014-05-23 /pmc/articles/PMC4172521/ /pubmed/24863242 http://dx.doi.org/10.1111/acel.12234 Text en © 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Takes Lackner, Daniel H Hayashi, Makoto T Cesare, Anthony J Karlseder, Jan A genomics approach identifies senescence-specific gene expression regulation |
title | A genomics approach identifies senescence-specific gene expression regulation |
title_full | A genomics approach identifies senescence-specific gene expression regulation |
title_fullStr | A genomics approach identifies senescence-specific gene expression regulation |
title_full_unstemmed | A genomics approach identifies senescence-specific gene expression regulation |
title_short | A genomics approach identifies senescence-specific gene expression regulation |
title_sort | genomics approach identifies senescence-specific gene expression regulation |
topic | Short Takes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172521/ https://www.ncbi.nlm.nih.gov/pubmed/24863242 http://dx.doi.org/10.1111/acel.12234 |
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