Cargando…
Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells
BACKGROUND: Ziziphora tenuior L. (Kakuti in Persian) is used in traditional medicine for treatment of gastrointestinal disorders as carminative and analgesic plant. The other usages of this plant are included treatment of diarrhea and nausea. Therefore in the present study we evaluated the immunomod...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172836/ https://www.ncbi.nlm.nih.gov/pubmed/25227582 http://dx.doi.org/10.1186/s40199-014-0063-8 |
_version_ | 1782336081233969152 |
---|---|
author | Azadmehr, Abbas latifi, Robabeh Mosalla, Sahar Hajiaghaee, Reza Shahnazi, Mojtaba |
author_facet | Azadmehr, Abbas latifi, Robabeh Mosalla, Sahar Hajiaghaee, Reza Shahnazi, Mojtaba |
author_sort | Azadmehr, Abbas |
collection | PubMed |
description | BACKGROUND: Ziziphora tenuior L. (Kakuti in Persian) is used in traditional medicine for treatment of gastrointestinal disorders as carminative and analgesic plant. The other usages of this plant are included treatment of diarrhea and nausea. Therefore in the present study we evaluated the immunomodulatory effects of the ethanolic extract of this plant on the dendritic cells (DCs). RESULTS: Ziziphora tenuior L. extract significantly (p = 0.002) increased the level of surface expression of CD40 as an important co-stimulatory marker on DCs compared to the control. However this extract did not change CD86 and MHC-II molecules, so it could promote DCs phenotypic maturation. Treatment of DCs with the extract resulted in slightly increased of the production of (IL-12); however, this change was not significant. In addition, the ability of treated DCs to stimulate allogenic T cells proliferation and cytokines secretion was examined in the co-cuture of these cells with T cells in mixed lymphocyte reaction (MLR). Z. tenuior L. at the 100 μg/ml concentration inhibited the proliferation of allogenic T cells and also significantly (P < 0.001) increased the level of IL-10. Moreover, the extract at 10–100 μg/ml concentration caused slightly increase in IFN-γ production and decreased IL-4 cytokines but these changes were not significant. CONCLUSIONS: These findings indicated that Z. tenuior L. extract can modulate immune response by induction of CD40 expression on DCs and cytokine production; whereas it can inhibit T cell stimulating activity of DCs in high concentration. These findings possibly in part explain the traditional use of this plant in treatment of immune-mediated disorders. However future studies are needed. |
format | Online Article Text |
id | pubmed-4172836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41728362014-09-25 Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells Azadmehr, Abbas latifi, Robabeh Mosalla, Sahar Hajiaghaee, Reza Shahnazi, Mojtaba Daru Research Article BACKGROUND: Ziziphora tenuior L. (Kakuti in Persian) is used in traditional medicine for treatment of gastrointestinal disorders as carminative and analgesic plant. The other usages of this plant are included treatment of diarrhea and nausea. Therefore in the present study we evaluated the immunomodulatory effects of the ethanolic extract of this plant on the dendritic cells (DCs). RESULTS: Ziziphora tenuior L. extract significantly (p = 0.002) increased the level of surface expression of CD40 as an important co-stimulatory marker on DCs compared to the control. However this extract did not change CD86 and MHC-II molecules, so it could promote DCs phenotypic maturation. Treatment of DCs with the extract resulted in slightly increased of the production of (IL-12); however, this change was not significant. In addition, the ability of treated DCs to stimulate allogenic T cells proliferation and cytokines secretion was examined in the co-cuture of these cells with T cells in mixed lymphocyte reaction (MLR). Z. tenuior L. at the 100 μg/ml concentration inhibited the proliferation of allogenic T cells and also significantly (P < 0.001) increased the level of IL-10. Moreover, the extract at 10–100 μg/ml concentration caused slightly increase in IFN-γ production and decreased IL-4 cytokines but these changes were not significant. CONCLUSIONS: These findings indicated that Z. tenuior L. extract can modulate immune response by induction of CD40 expression on DCs and cytokine production; whereas it can inhibit T cell stimulating activity of DCs in high concentration. These findings possibly in part explain the traditional use of this plant in treatment of immune-mediated disorders. However future studies are needed. BioMed Central 2014-09-17 /pmc/articles/PMC4172836/ /pubmed/25227582 http://dx.doi.org/10.1186/s40199-014-0063-8 Text en © Azadmehr et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Azadmehr, Abbas latifi, Robabeh Mosalla, Sahar Hajiaghaee, Reza Shahnazi, Mojtaba Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title | Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title_full | Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title_fullStr | Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title_full_unstemmed | Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title_short | Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells |
title_sort | immunomodulatory effects of ziziphora tenuior l. extract on the dendritic cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172836/ https://www.ncbi.nlm.nih.gov/pubmed/25227582 http://dx.doi.org/10.1186/s40199-014-0063-8 |
work_keys_str_mv | AT azadmehrabbas immunomodulatoryeffectsofziziphoratenuiorlextractonthedendriticcells AT latifirobabeh immunomodulatoryeffectsofziziphoratenuiorlextractonthedendriticcells AT mosallasahar immunomodulatoryeffectsofziziphoratenuiorlextractonthedendriticcells AT hajiaghaeereza immunomodulatoryeffectsofziziphoratenuiorlextractonthedendriticcells AT shahnazimojtaba immunomodulatoryeffectsofziziphoratenuiorlextractonthedendriticcells |