High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

BACKGROUND: α-2,6-sialyltransferase catalyzes the terminal step of complex N-glycan biosynthesis on human glycoproteins, attaching sialic acid to outermost galactosyl residues on otherwise fully assembled branched glycans. This “capping” of N-glycans is critical for therapeutic efficacy of pharmaceu...

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Detalles Bibliográficos
Autores principales: Ribitsch, Doris, Zitzenbacher, Sabine, Augustin, Peter, Schmölzer, Katharina, Czabany, Tibor, Luley-Goedl, Christiane, Thomann, Marco, Jung, Christine, Sobek, Harald, Müller, Rainer, Nidetzky, Bernd, Schwab, Helmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172862/
https://www.ncbi.nlm.nih.gov/pubmed/25365915
http://dx.doi.org/10.1186/s12934-014-0138-8

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