Cargando…

Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation

BACKGROUND: Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of ana...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghazali, M Faizal, Koh-Tan, HH Caline, McLaughlin, Mark, Montague, Paul, Jonsson, Nicholas N, Eckersall, P David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4173004/
https://www.ncbi.nlm.nih.gov/pubmed/25927913
http://dx.doi.org/10.1186/s12917-014-0204-9
_version_ 1782336119820517376
author Ghazali, M Faizal
Koh-Tan, HH Caline
McLaughlin, Mark
Montague, Paul
Jonsson, Nicholas N
Eckersall, P David
author_facet Ghazali, M Faizal
Koh-Tan, HH Caline
McLaughlin, Mark
Montague, Paul
Jonsson, Nicholas N
Eckersall, P David
author_sort Ghazali, M Faizal
collection PubMed
description BACKGROUND: Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of analytes that are present in the NS of healthy cattle. Biochemical profiles of NS from a group of 38 healthy Holstein-Friesian cows revealed high alkaline phosphatase (AP) activity of up to 2392 IU/L. The character and source of the high activity of AP in bovine NS was investigated. RESULTS: Histochemical analysis confirmed the localization of the AP enzyme activity to epithelial cells and serous glands of the nasal respiratory mucosa. Analysis of mRNA levels from nasal mucosa by end point RT-PCR and PCR product sequencing confirmed that the AP was locally produced and is identical at the nucleotide level to the non-specific AP splice variant found in bovine liver, bone and kidney. Analysis by isoelectric focussing confirmed that AP was produced locally at a high level in nasal epithelium demonstrating that AP from nasal secretion and nasal mucosa had similar pI bands, though differing from those of the liver, kidney, bone and intestine, suggesting different post-translational modification (PTM) of AP in these tissues. CONCLUSIONS: A nasal isozyme of AP has been identified that is present at a high activity in NS, resulting from local production and showing distinctive PTM and may be active in NS as an anti-endotoxin mediator.
format Online
Article
Text
id pubmed-4173004
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-41730042014-09-25 Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation Ghazali, M Faizal Koh-Tan, HH Caline McLaughlin, Mark Montague, Paul Jonsson, Nicholas N Eckersall, P David BMC Vet Res Research Article BACKGROUND: Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of analytes that are present in the NS of healthy cattle. Biochemical profiles of NS from a group of 38 healthy Holstein-Friesian cows revealed high alkaline phosphatase (AP) activity of up to 2392 IU/L. The character and source of the high activity of AP in bovine NS was investigated. RESULTS: Histochemical analysis confirmed the localization of the AP enzyme activity to epithelial cells and serous glands of the nasal respiratory mucosa. Analysis of mRNA levels from nasal mucosa by end point RT-PCR and PCR product sequencing confirmed that the AP was locally produced and is identical at the nucleotide level to the non-specific AP splice variant found in bovine liver, bone and kidney. Analysis by isoelectric focussing confirmed that AP was produced locally at a high level in nasal epithelium demonstrating that AP from nasal secretion and nasal mucosa had similar pI bands, though differing from those of the liver, kidney, bone and intestine, suggesting different post-translational modification (PTM) of AP in these tissues. CONCLUSIONS: A nasal isozyme of AP has been identified that is present at a high activity in NS, resulting from local production and showing distinctive PTM and may be active in NS as an anti-endotoxin mediator. BioMed Central 2014-09-05 /pmc/articles/PMC4173004/ /pubmed/25927913 http://dx.doi.org/10.1186/s12917-014-0204-9 Text en © Ghazali et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ghazali, M Faizal
Koh-Tan, HH Caline
McLaughlin, Mark
Montague, Paul
Jonsson, Nicholas N
Eckersall, P David
Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title_full Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title_fullStr Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title_full_unstemmed Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title_short Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
title_sort alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4173004/
https://www.ncbi.nlm.nih.gov/pubmed/25927913
http://dx.doi.org/10.1186/s12917-014-0204-9
work_keys_str_mv AT ghazalimfaizal alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation
AT kohtanhhcaline alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation
AT mclaughlinmark alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation
AT montaguepaul alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation
AT jonssonnicholasn alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation
AT eckersallpdavid alkalinephosphataseinnasalsecretionofcattlebiochemicalandmolecularcharacterisation