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Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft

BACKGROUND: Hyperbaric oxygenation has been used for various purposes, but its clinical application is limited due to its pulmonary toxicity. We evaluated the therapeutic value of normobaric hyperoxygenation (NBO) for vascularized and nonvascularized tissue transplantation. METHODS: Tissue oxygen pa...

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Autores principales: Araki, Jun, Kato, Harunosuke, Doi, Kentaro, Kuno, Shinichiro, Kinoshita, Kahori, Mineda, Kazuhide, Kanayama, Koji, Yoshimura, Kotaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174081/
https://www.ncbi.nlm.nih.gov/pubmed/25289345
http://dx.doi.org/10.1097/GOX.0000000000000029
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author Araki, Jun
Kato, Harunosuke
Doi, Kentaro
Kuno, Shinichiro
Kinoshita, Kahori
Mineda, Kazuhide
Kanayama, Koji
Yoshimura, Kotaro
author_facet Araki, Jun
Kato, Harunosuke
Doi, Kentaro
Kuno, Shinichiro
Kinoshita, Kahori
Mineda, Kazuhide
Kanayama, Koji
Yoshimura, Kotaro
author_sort Araki, Jun
collection PubMed
description BACKGROUND: Hyperbaric oxygenation has been used for various purposes, but its clinical application is limited due to its pulmonary toxicity. We evaluated the therapeutic value of normobaric hyperoxygenation (NBO) for vascularized and nonvascularized tissue transplantation. METHODS: Tissue oxygen partial pressure (PtO(2)) was measured for various organs in mice under inspiratory oxygen of 20%, 60%, or 100%. A rectangular skin flap (1 × 4 cm) or a composite skin graft (2 × 2 cm) was made on the back of mice, which were housed under 20% or 60% oxygen for the first 3 days after surgery. Cell survival was also examined in organ culture skin samples. RESULTS: PtO(2) varied among tissues/organs, but increased depending on inspiratory oxygen concentration in all tissues/organs. Although NBO with 100% O(2) was toxic, NBO with 60% O(2) was safe even when used continuously for a long period. NBO did not significantly improve survival of the rectangular skin flap. On the other hand, in the composite skin graft model, the engraftment area increased significantly (52 ± 10 at 20% vs 68 ± 5.1 at 60%) and contraction decreased significantly (42 ± 8.0 at 20% vs 27 ± 5.7 at 60%). Organ culture of a composite skin sample showed significant cell death under lower oxygen concentrations, supporting the data in vivo. CONCLUSIONS: The composite graft was maintained until revascularization by plasmatic diffusion from surrounding tissues, in which PtO(2) was improved by NBO. NBO may be an effective adjunct therapy that can be performed readily after nonvascularized tissue grafting.
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spelling pubmed-41740812014-10-06 Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft Araki, Jun Kato, Harunosuke Doi, Kentaro Kuno, Shinichiro Kinoshita, Kahori Mineda, Kazuhide Kanayama, Koji Yoshimura, Kotaro Plast Reconstr Surg Glob Open Experimental BACKGROUND: Hyperbaric oxygenation has been used for various purposes, but its clinical application is limited due to its pulmonary toxicity. We evaluated the therapeutic value of normobaric hyperoxygenation (NBO) for vascularized and nonvascularized tissue transplantation. METHODS: Tissue oxygen partial pressure (PtO(2)) was measured for various organs in mice under inspiratory oxygen of 20%, 60%, or 100%. A rectangular skin flap (1 × 4 cm) or a composite skin graft (2 × 2 cm) was made on the back of mice, which were housed under 20% or 60% oxygen for the first 3 days after surgery. Cell survival was also examined in organ culture skin samples. RESULTS: PtO(2) varied among tissues/organs, but increased depending on inspiratory oxygen concentration in all tissues/organs. Although NBO with 100% O(2) was toxic, NBO with 60% O(2) was safe even when used continuously for a long period. NBO did not significantly improve survival of the rectangular skin flap. On the other hand, in the composite skin graft model, the engraftment area increased significantly (52 ± 10 at 20% vs 68 ± 5.1 at 60%) and contraction decreased significantly (42 ± 8.0 at 20% vs 27 ± 5.7 at 60%). Organ culture of a composite skin sample showed significant cell death under lower oxygen concentrations, supporting the data in vivo. CONCLUSIONS: The composite graft was maintained until revascularization by plasmatic diffusion from surrounding tissues, in which PtO(2) was improved by NBO. NBO may be an effective adjunct therapy that can be performed readily after nonvascularized tissue grafting. Wolters Kluwer Health 2014-06-06 /pmc/articles/PMC4174081/ /pubmed/25289345 http://dx.doi.org/10.1097/GOX.0000000000000029 Text en Copyright © 2014 The Authors. Published by Lippincott Williams & Wilkins on behalf of The American Society of Plastic Surgeons. PRS Global Open is a publication of the American Society of Plastic Surgeons. http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
spellingShingle Experimental
Araki, Jun
Kato, Harunosuke
Doi, Kentaro
Kuno, Shinichiro
Kinoshita, Kahori
Mineda, Kazuhide
Kanayama, Koji
Yoshimura, Kotaro
Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title_full Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title_fullStr Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title_full_unstemmed Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title_short Application of Normobaric Hyperoxygenation to an Ischemic Flap and a Composite Skin Graft
title_sort application of normobaric hyperoxygenation to an ischemic flap and a composite skin graft
topic Experimental
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174081/
https://www.ncbi.nlm.nih.gov/pubmed/25289345
http://dx.doi.org/10.1097/GOX.0000000000000029
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