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Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics

[Image: see text] Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT to...

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Autores principales: Silva, Rona M., Doudrick, Kyle, Franzi, Lisa M., TeeSy, Christel, Anderson, Donald S., Wu, Zheqiong, Mitra, Somenath, Vu, Vincent, Dutrow, Gavin, Evans, James E., Westerhoff, Paul, Van Winkle, Laura S., Raabe, Otto G., Pinkerton, Kent E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174094/
https://www.ncbi.nlm.nih.gov/pubmed/25144856
http://dx.doi.org/10.1021/nn503887r
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author Silva, Rona M.
Doudrick, Kyle
Franzi, Lisa M.
TeeSy, Christel
Anderson, Donald S.
Wu, Zheqiong
Mitra, Somenath
Vu, Vincent
Dutrow, Gavin
Evans, James E.
Westerhoff, Paul
Van Winkle, Laura S.
Raabe, Otto G.
Pinkerton, Kent E.
author_facet Silva, Rona M.
Doudrick, Kyle
Franzi, Lisa M.
TeeSy, Christel
Anderson, Donald S.
Wu, Zheqiong
Mitra, Somenath
Vu, Vincent
Dutrow, Gavin
Evans, James E.
Westerhoff, Paul
Van Winkle, Laura S.
Raabe, Otto G.
Pinkerton, Kent E.
author_sort Silva, Rona M.
collection PubMed
description [Image: see text] Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT toxicity with the hypothesis that these factors will influence significantly different responses upon MWCNT exposure. The study is unique in that (1) multiple administration methods were tested using particles from the same stock; (2) bulk MWCNT formulations had few differences (metal content, surface area/functionalization); and (3) MWCNT retention was quantified using a specialized approach for measuring unlabeled MWCNTs in rodent lungs. Male Sprague–Dawley rats were exposed to original (O), purified (P), and carboxylic acid functionalized (F) MWCNTs via intratracheal instillation and inhalation. Blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected at postexposure days 1 and 21 for quantifying biological responses and MWCNTs in lung tissues by programmed thermal analysis. At day 1, MWCNT instillation produced significant BALF neutrophilia and MWCNT-positive macrophages. Instilled O- and P-MWCNTs produced significant inflammation in lung tissues, which resolved by day 21 despite MWCNT retention. MWCNT inhalation produced no BALF neutrophilia and no significant histopathology past day 1. However, on days 1 and 21 postinhalation of nebulized MWCNTs, significantly increased numbers of MWCNT-positive macrophages were observed in BALF. Results suggest (1) MWCNTs produce transient inflammation if any despite persistence in the lungs; (2) instilled O-MWCNTs cause more inflammation than P- or F-MWCNTs; and (3) MWCNT suspension media produce strikingly different effects on physicochemical particle characteristics and pulmonary responses.
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spelling pubmed-41740942015-08-14 Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics Silva, Rona M. Doudrick, Kyle Franzi, Lisa M. TeeSy, Christel Anderson, Donald S. Wu, Zheqiong Mitra, Somenath Vu, Vincent Dutrow, Gavin Evans, James E. Westerhoff, Paul Van Winkle, Laura S. Raabe, Otto G. Pinkerton, Kent E. ACS Nano [Image: see text] Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT toxicity with the hypothesis that these factors will influence significantly different responses upon MWCNT exposure. The study is unique in that (1) multiple administration methods were tested using particles from the same stock; (2) bulk MWCNT formulations had few differences (metal content, surface area/functionalization); and (3) MWCNT retention was quantified using a specialized approach for measuring unlabeled MWCNTs in rodent lungs. Male Sprague–Dawley rats were exposed to original (O), purified (P), and carboxylic acid functionalized (F) MWCNTs via intratracheal instillation and inhalation. Blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected at postexposure days 1 and 21 for quantifying biological responses and MWCNTs in lung tissues by programmed thermal analysis. At day 1, MWCNT instillation produced significant BALF neutrophilia and MWCNT-positive macrophages. Instilled O- and P-MWCNTs produced significant inflammation in lung tissues, which resolved by day 21 despite MWCNT retention. MWCNT inhalation produced no BALF neutrophilia and no significant histopathology past day 1. However, on days 1 and 21 postinhalation of nebulized MWCNTs, significantly increased numbers of MWCNT-positive macrophages were observed in BALF. Results suggest (1) MWCNTs produce transient inflammation if any despite persistence in the lungs; (2) instilled O-MWCNTs cause more inflammation than P- or F-MWCNTs; and (3) MWCNT suspension media produce strikingly different effects on physicochemical particle characteristics and pulmonary responses. American Chemical Society 2014-08-14 2014-09-23 /pmc/articles/PMC4174094/ /pubmed/25144856 http://dx.doi.org/10.1021/nn503887r Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Silva, Rona M.
Doudrick, Kyle
Franzi, Lisa M.
TeeSy, Christel
Anderson, Donald S.
Wu, Zheqiong
Mitra, Somenath
Vu, Vincent
Dutrow, Gavin
Evans, James E.
Westerhoff, Paul
Van Winkle, Laura S.
Raabe, Otto G.
Pinkerton, Kent E.
Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title_full Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title_fullStr Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title_full_unstemmed Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title_short Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
title_sort instillation versus inhalation of multiwalled carbon nanotubes: exposure-related health effects, clearance, and the role of particle characteristics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174094/
https://www.ncbi.nlm.nih.gov/pubmed/25144856
http://dx.doi.org/10.1021/nn503887r
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