Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation

[Image: see text] Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or m...

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Autores principales: Rouge, Jessica L., Hao, Liangliang, Wu, Xiaochen A., Briley, William E., Mirkin, Chad A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174098/
https://www.ncbi.nlm.nih.gov/pubmed/25144723
http://dx.doi.org/10.1021/nn503601s
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author Rouge, Jessica L.
Hao, Liangliang
Wu, Xiaochen A.
Briley, William E.
Mirkin, Chad A.
author_facet Rouge, Jessica L.
Hao, Liangliang
Wu, Xiaochen A.
Briley, William E.
Mirkin, Chad A.
author_sort Rouge, Jessica L.
collection PubMed
description [Image: see text] Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA–nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.
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spelling pubmed-41740982015-08-21 Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation Rouge, Jessica L. Hao, Liangliang Wu, Xiaochen A. Briley, William E. Mirkin, Chad A. ACS Nano [Image: see text] Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA–nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct. American Chemical Society 2014-08-21 2014-09-23 /pmc/articles/PMC4174098/ /pubmed/25144723 http://dx.doi.org/10.1021/nn503601s Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Rouge, Jessica L.
Hao, Liangliang
Wu, Xiaochen A.
Briley, William E.
Mirkin, Chad A.
Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title_full Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title_fullStr Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title_full_unstemmed Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title_short Spherical Nucleic Acids as a Divergent Platform for Synthesizing RNA–Nanoparticle Conjugates through Enzymatic Ligation
title_sort spherical nucleic acids as a divergent platform for synthesizing rna–nanoparticle conjugates through enzymatic ligation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174098/
https://www.ncbi.nlm.nih.gov/pubmed/25144723
http://dx.doi.org/10.1021/nn503601s
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