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Transcriptionally Profiling Cells Sorted by Transcript Abundance
We have developed a quantitative technique for sorting cells based on endogenous RNA abundance with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high fidelity transcriptome measurement of (mouse) in...
Autores principales: | Klemm, Sandy, Semrau, Stefan, Wiebrands, Kay, Mooijman, Dylan, Faddah, Dina A., Jaenisch, Rudolf, van Oudenaarden, Alexander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174458/ https://www.ncbi.nlm.nih.gov/pubmed/24681693 http://dx.doi.org/10.1038/nmeth.2910 |
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