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Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients
BACKGROUND: Inflammation is an essential immune response; however, chronic inflammation results in disease including Crohn's disease. Therefore, reducing the inflammation can yield a significant health benefit, and one way to achieve this is through diet. We developed a Mediterranean-inspired a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174666/ https://www.ncbi.nlm.nih.gov/pubmed/24283712 http://dx.doi.org/10.1186/1479-7364-7-24 |
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author | Marlow, Gareth Ellett, Stephanie Ferguson, Isobel R Zhu, Shuotun Karunasinghe, Nishi Jesuthasan, Amalini C Han, Dug Yeo Fraser, Alan G Ferguson, Lynnette R |
author_facet | Marlow, Gareth Ellett, Stephanie Ferguson, Isobel R Zhu, Shuotun Karunasinghe, Nishi Jesuthasan, Amalini C Han, Dug Yeo Fraser, Alan G Ferguson, Lynnette R |
author_sort | Marlow, Gareth |
collection | PubMed |
description | BACKGROUND: Inflammation is an essential immune response; however, chronic inflammation results in disease including Crohn's disease. Therefore, reducing the inflammation can yield a significant health benefit, and one way to achieve this is through diet. We developed a Mediterranean-inspired anti-inflammatory diet and used this diet in a 6-week intervention in a Crohn's disease population. We examined changes in inflammation and also in the gut microbiota. We compared the results of established biomarkers, C-reactive protein and the micronuclei assay, of inflammation with results from a transcriptomic approach. RESULTS: Data showed that being on our diet for 6 weeks was able to reduce the established biomarkers of inflammation. However, using transcriptomics, we observed significant changes in gene expression. Although no single gene stood out, the cumulative effect of small changes in many genes combined to have a beneficial effect. Data also showed that our diet resulted in a trend of normalising the microbiota. CONCLUSIONS: This study showed that our Mediterranean-inspired diet appeared to benefit the health of people with Crohn's disease. Our participants showed a trend for reduced markers of inflammation and normalising of the microbiota. The significant changes in gene expression after 6 weeks highlighted the increased sensitivity of using transcriptomics when compared to the established biomarkers and open up a new era of dietary intervention studies. |
format | Online Article Text |
id | pubmed-4174666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41746662014-09-26 Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients Marlow, Gareth Ellett, Stephanie Ferguson, Isobel R Zhu, Shuotun Karunasinghe, Nishi Jesuthasan, Amalini C Han, Dug Yeo Fraser, Alan G Ferguson, Lynnette R Hum Genomics Primary Research BACKGROUND: Inflammation is an essential immune response; however, chronic inflammation results in disease including Crohn's disease. Therefore, reducing the inflammation can yield a significant health benefit, and one way to achieve this is through diet. We developed a Mediterranean-inspired anti-inflammatory diet and used this diet in a 6-week intervention in a Crohn's disease population. We examined changes in inflammation and also in the gut microbiota. We compared the results of established biomarkers, C-reactive protein and the micronuclei assay, of inflammation with results from a transcriptomic approach. RESULTS: Data showed that being on our diet for 6 weeks was able to reduce the established biomarkers of inflammation. However, using transcriptomics, we observed significant changes in gene expression. Although no single gene stood out, the cumulative effect of small changes in many genes combined to have a beneficial effect. Data also showed that our diet resulted in a trend of normalising the microbiota. CONCLUSIONS: This study showed that our Mediterranean-inspired diet appeared to benefit the health of people with Crohn's disease. Our participants showed a trend for reduced markers of inflammation and normalising of the microbiota. The significant changes in gene expression after 6 weeks highlighted the increased sensitivity of using transcriptomics when compared to the established biomarkers and open up a new era of dietary intervention studies. BioMed Central 2013-11-27 /pmc/articles/PMC4174666/ /pubmed/24283712 http://dx.doi.org/10.1186/1479-7364-7-24 Text en Copyright © 2013 Marlow et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Primary Research Marlow, Gareth Ellett, Stephanie Ferguson, Isobel R Zhu, Shuotun Karunasinghe, Nishi Jesuthasan, Amalini C Han, Dug Yeo Fraser, Alan G Ferguson, Lynnette R Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title | Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title_full | Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title_fullStr | Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title_full_unstemmed | Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title_short | Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients |
title_sort | transcriptomics to study the effect of a mediterranean-inspired diet on inflammation in crohn's disease patients |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174666/ https://www.ncbi.nlm.nih.gov/pubmed/24283712 http://dx.doi.org/10.1186/1479-7364-7-24 |
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