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Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1

Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 we...

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Autores principales: Elsharkawy, Mohsen Mohamed, Shimizu, Masafumi, Takahashi, Hideki, Ozaki, Kouichi, Hyakumachi, Mitsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174775/
https://www.ncbi.nlm.nih.gov/pubmed/25288946
http://dx.doi.org/10.5423/PPJ.SI.07.2012.01
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author Elsharkawy, Mohsen Mohamed
Shimizu, Masafumi
Takahashi, Hideki
Ozaki, Kouichi
Hyakumachi, Mitsuro
author_facet Elsharkawy, Mohsen Mohamed
Shimizu, Masafumi
Takahashi, Hideki
Ozaki, Kouichi
Hyakumachi, Mitsuro
author_sort Elsharkawy, Mohsen Mohamed
collection PubMed
description Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.
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spelling pubmed-41747752014-10-06 Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1 Elsharkawy, Mohsen Mohamed Shimizu, Masafumi Takahashi, Hideki Ozaki, Kouichi Hyakumachi, Mitsuro Plant Pathol J Articles Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection. Korean Society of Plant Pathology 2013-06 /pmc/articles/PMC4174775/ /pubmed/25288946 http://dx.doi.org/10.5423/PPJ.SI.07.2012.01 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Elsharkawy, Mohsen Mohamed
Shimizu, Masafumi
Takahashi, Hideki
Ozaki, Kouichi
Hyakumachi, Mitsuro
Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title_full Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title_fullStr Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title_full_unstemmed Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title_short Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1
title_sort induction of systemic resistance against cucumber mosaic virus in arabidopsis thaliana by trichoderma asperellum skt-1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174775/
https://www.ncbi.nlm.nih.gov/pubmed/25288946
http://dx.doi.org/10.5423/PPJ.SI.07.2012.01
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