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Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice

Plant has possessed diverse stress signals from outside and maintained its fitness. Out of such plant responses, it is well known that mitogen-activated protein kinase (MAPK) cascade plays important role in wounding and pathogen attack in most dicot plants. However, little is understood about its ro...

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Autores principales: Yoo, Seung Jin, Kim, Su-Hyun, Kim, Min-Jeong, Ryu, Choong-Min, Kim, Young Cheol, Cho, Baik Ho, Yang, Kwang-Yeol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174855/
https://www.ncbi.nlm.nih.gov/pubmed/25288999
http://dx.doi.org/10.5423/PPJ.OA.10.2013.0106
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author Yoo, Seung Jin
Kim, Su-Hyun
Kim, Min-Jeong
Ryu, Choong-Min
Kim, Young Cheol
Cho, Baik Ho
Yang, Kwang-Yeol
author_facet Yoo, Seung Jin
Kim, Su-Hyun
Kim, Min-Jeong
Ryu, Choong-Min
Kim, Young Cheol
Cho, Baik Ho
Yang, Kwang-Yeol
author_sort Yoo, Seung Jin
collection PubMed
description Plant has possessed diverse stress signals from outside and maintained its fitness. Out of such plant responses, it is well known that mitogen-activated protein kinase (MAPK) cascade plays important role in wounding and pathogen attack in most dicot plants. However, little is understood about its role in wounding response for the economically important monocot rice plant. In this study, therefore, the involvement of MAPK was investigated to understand the wounding signaling pathway in rice. The OsMPK1 was rapidly activated by wounding within 10 min, and OsMPK1 was also activated by challenge of rice blast fungus. Further analysis revealed that OsMKK4, the upstream kinase of OsMPK1, phosphorylated OsMPK1 by wounding in vivo. Furthermore, OsMPK1 directly interacted with a rice defense-related transcription factor OsWRKY53. To understand a functional link between MAPK and its target transcription factor, we showed that OsMPK1 activated by the constitutively active mutant OsMKK4(DD) phosphorylated OsWRKY53 in vitro. Taken together, components involving in the wounding signaling pathway, OsMKK4-OsMPK1-OsWRKY53, can be important players in regulating crosstalk between abiotic stress and biotic stress.
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spelling pubmed-41748552014-10-06 Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice Yoo, Seung Jin Kim, Su-Hyun Kim, Min-Jeong Ryu, Choong-Min Kim, Young Cheol Cho, Baik Ho Yang, Kwang-Yeol Plant Pathol J Research Article Plant has possessed diverse stress signals from outside and maintained its fitness. Out of such plant responses, it is well known that mitogen-activated protein kinase (MAPK) cascade plays important role in wounding and pathogen attack in most dicot plants. However, little is understood about its role in wounding response for the economically important monocot rice plant. In this study, therefore, the involvement of MAPK was investigated to understand the wounding signaling pathway in rice. The OsMPK1 was rapidly activated by wounding within 10 min, and OsMPK1 was also activated by challenge of rice blast fungus. Further analysis revealed that OsMKK4, the upstream kinase of OsMPK1, phosphorylated OsMPK1 by wounding in vivo. Furthermore, OsMPK1 directly interacted with a rice defense-related transcription factor OsWRKY53. To understand a functional link between MAPK and its target transcription factor, we showed that OsMPK1 activated by the constitutively active mutant OsMKK4(DD) phosphorylated OsWRKY53 in vitro. Taken together, components involving in the wounding signaling pathway, OsMKK4-OsMPK1-OsWRKY53, can be important players in regulating crosstalk between abiotic stress and biotic stress. Korean Society of Plant Pathology 2014-06 /pmc/articles/PMC4174855/ /pubmed/25288999 http://dx.doi.org/10.5423/PPJ.OA.10.2013.0106 Text en ©The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yoo, Seung Jin
Kim, Su-Hyun
Kim, Min-Jeong
Ryu, Choong-Min
Kim, Young Cheol
Cho, Baik Ho
Yang, Kwang-Yeol
Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title_full Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title_fullStr Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title_full_unstemmed Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title_short Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice
title_sort involvement of the osmkk4-osmpk1 cascade and its downstream transcription factor oswrky53 in the wounding response in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174855/
https://www.ncbi.nlm.nih.gov/pubmed/25288999
http://dx.doi.org/10.5423/PPJ.OA.10.2013.0106
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