Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells

We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These d...

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Autores principales: Chang, Mi-Yoon, Rhee, Yong-Hee, Yi, Sang-Hoon, Lee, Su-Jae, Kim, Rae-Kwon, Kim, Hyongbum, Park, Chang-Hwan, Lee, Sang-Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175555/
https://www.ncbi.nlm.nih.gov/pubmed/25254347
http://dx.doi.org/10.1016/j.stemcr.2014.06.013
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author Chang, Mi-Yoon
Rhee, Yong-Hee
Yi, Sang-Hoon
Lee, Su-Jae
Kim, Rae-Kwon
Kim, Hyongbum
Park, Chang-Hwan
Lee, Sang-Hun
author_facet Chang, Mi-Yoon
Rhee, Yong-Hee
Yi, Sang-Hoon
Lee, Su-Jae
Kim, Rae-Kwon
Kim, Hyongbum
Park, Chang-Hwan
Lee, Sang-Hun
author_sort Chang, Mi-Yoon
collection PubMed
description We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.
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spelling pubmed-41755552014-09-30 Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells Chang, Mi-Yoon Rhee, Yong-Hee Yi, Sang-Hoon Lee, Su-Jae Kim, Rae-Kwon Kim, Hyongbum Park, Chang-Hwan Lee, Sang-Hun Stem Cell Reports Article We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance. Elsevier 2014-07-24 /pmc/articles/PMC4175555/ /pubmed/25254347 http://dx.doi.org/10.1016/j.stemcr.2014.06.013 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Chang, Mi-Yoon
Rhee, Yong-Hee
Yi, Sang-Hoon
Lee, Su-Jae
Kim, Rae-Kwon
Kim, Hyongbum
Park, Chang-Hwan
Lee, Sang-Hun
Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title_full Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title_fullStr Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title_full_unstemmed Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title_short Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells
title_sort doxycycline enhances survival and self-renewal of human pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175555/
https://www.ncbi.nlm.nih.gov/pubmed/25254347
http://dx.doi.org/10.1016/j.stemcr.2014.06.013
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