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Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats

INTRODUCTION: Acute pancreatitis (AP) is known to induce injuries to extrapancreatic organs. Because respiratory dysfunction is the main cause of death in patients with severe AP, acute pancreatitis-associated lung injury (APALI) is a great challenge for clinicians. This study aimed to investigate t...

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Autores principales: Qu, Zhen, Jiang, Yong, Wu, Bao-Qiang, Duan, Yun-Fei, Sun, Zhen-Di, Luo, Guang-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175783/
https://www.ncbi.nlm.nih.gov/pubmed/25276170
http://dx.doi.org/10.5114/aoms.2014.44873
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author Qu, Zhen
Jiang, Yong
Wu, Bao-Qiang
Duan, Yun-Fei
Sun, Zhen-Di
Luo, Guang-Hua
author_facet Qu, Zhen
Jiang, Yong
Wu, Bao-Qiang
Duan, Yun-Fei
Sun, Zhen-Di
Luo, Guang-Hua
author_sort Qu, Zhen
collection PubMed
description INTRODUCTION: Acute pancreatitis (AP) is known to induce injuries to extrapancreatic organs. Because respiratory dysfunction is the main cause of death in patients with severe AP, acute pancreatitis-associated lung injury (APALI) is a great challenge for clinicians. This study aimed to investigate the potential role of hydrogen sulfide (H(2)S) in the pathogenesis of APALI. MATERIAL AND METHODS: Fifty-four SD rats were randomly divided into three groups: the AP group of rats that received injection of sodium deoxycholate into the common bile duct, the control group that underwent a sham operation, and the treatment group made by intraperitoneal injection of propargylglycine (PAG), an inhibitor of cystathionine-γ-lyase (CSE), into rats with AP. Histopathology of the lung was examined and the expression of CSE and TNF-α mRNA in lung tissue was detected by real-time polymerase chain reaction. The H(2)S level in the serum was detected spectrophotometrically. RESULTS: The serum concentration of H2S and CSE and TNF-α expression in the lung were increased in AP rats modeled after 3 h and 6 h than in control rats (p < 0.05). Intraperitoneal injection of PAG could reduce the serum concentration of H(2)S, reduce CSE and TNF-α expression, and alleviate the lung pathology (p < 0.05). CONCLUSIONS: Taken together, our findings suggest that the H(2)S/CSE system is crucially involved in the pathological process of APALI and represents a novel target for the therapy of APALI.
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spelling pubmed-41757832014-09-30 Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats Qu, Zhen Jiang, Yong Wu, Bao-Qiang Duan, Yun-Fei Sun, Zhen-Di Luo, Guang-Hua Arch Med Sci Experimental Research INTRODUCTION: Acute pancreatitis (AP) is known to induce injuries to extrapancreatic organs. Because respiratory dysfunction is the main cause of death in patients with severe AP, acute pancreatitis-associated lung injury (APALI) is a great challenge for clinicians. This study aimed to investigate the potential role of hydrogen sulfide (H(2)S) in the pathogenesis of APALI. MATERIAL AND METHODS: Fifty-four SD rats were randomly divided into three groups: the AP group of rats that received injection of sodium deoxycholate into the common bile duct, the control group that underwent a sham operation, and the treatment group made by intraperitoneal injection of propargylglycine (PAG), an inhibitor of cystathionine-γ-lyase (CSE), into rats with AP. Histopathology of the lung was examined and the expression of CSE and TNF-α mRNA in lung tissue was detected by real-time polymerase chain reaction. The H(2)S level in the serum was detected spectrophotometrically. RESULTS: The serum concentration of H2S and CSE and TNF-α expression in the lung were increased in AP rats modeled after 3 h and 6 h than in control rats (p < 0.05). Intraperitoneal injection of PAG could reduce the serum concentration of H(2)S, reduce CSE and TNF-α expression, and alleviate the lung pathology (p < 0.05). CONCLUSIONS: Taken together, our findings suggest that the H(2)S/CSE system is crucially involved in the pathological process of APALI and represents a novel target for the therapy of APALI. Termedia Publishing House 2014-08-29 2014-08-29 /pmc/articles/PMC4175783/ /pubmed/25276170 http://dx.doi.org/10.5114/aoms.2014.44873 Text en Copyright © 2014 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Experimental Research
Qu, Zhen
Jiang, Yong
Wu, Bao-Qiang
Duan, Yun-Fei
Sun, Zhen-Di
Luo, Guang-Hua
Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title_full Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title_fullStr Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title_full_unstemmed Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title_short Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
title_sort cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats
topic Experimental Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4175783/
https://www.ncbi.nlm.nih.gov/pubmed/25276170
http://dx.doi.org/10.5114/aoms.2014.44873
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