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Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach

Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. D...

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Autores principales: Cluitmans, Judith C. A., Chokkalingam, Venkatachalam, Janssen, Arno M., Brock, Roland, Huck, Wilhelm T. S., Bosman, Giel J. C. G. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176636/
https://www.ncbi.nlm.nih.gov/pubmed/25295273
http://dx.doi.org/10.1155/2014/764268
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author Cluitmans, Judith C. A.
Chokkalingam, Venkatachalam
Janssen, Arno M.
Brock, Roland
Huck, Wilhelm T. S.
Bosman, Giel J. C. G. M.
author_facet Cluitmans, Judith C. A.
Chokkalingam, Venkatachalam
Janssen, Arno M.
Brock, Roland
Huck, Wilhelm T. S.
Bosman, Giel J. C. G. M.
author_sort Cluitmans, Judith C. A.
collection PubMed
description Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. During blood bank storage and in many pathophysiological conditions, RBC morphology changes, which has been suggested to be associated with decreased deformability and removal of RBC. While various techniques provide information on the rheological properties of stored RBCs, their clinical significance is controversial. We developed a microfluidic approach for evaluating RBC deformability in a physiologically meaningful and clinically significant manner. Unlike other techniques, our method enables a high-throughput determination of changes in deformation capacity to provide statistically significant data, while providing morphological information at the single-cell level. Our data show that, under conditions that closely mimic capillary dimensions and flow, the capacity to deform and the capacity to relax are not affected during storage in the blood bank. Our data also show that altered cell morphology by itself does not necessarily affect deformability.
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spelling pubmed-41766362014-10-07 Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach Cluitmans, Judith C. A. Chokkalingam, Venkatachalam Janssen, Arno M. Brock, Roland Huck, Wilhelm T. S. Bosman, Giel J. C. G. M. Biomed Res Int Research Article Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. During blood bank storage and in many pathophysiological conditions, RBC morphology changes, which has been suggested to be associated with decreased deformability and removal of RBC. While various techniques provide information on the rheological properties of stored RBCs, their clinical significance is controversial. We developed a microfluidic approach for evaluating RBC deformability in a physiologically meaningful and clinically significant manner. Unlike other techniques, our method enables a high-throughput determination of changes in deformation capacity to provide statistically significant data, while providing morphological information at the single-cell level. Our data show that, under conditions that closely mimic capillary dimensions and flow, the capacity to deform and the capacity to relax are not affected during storage in the blood bank. Our data also show that altered cell morphology by itself does not necessarily affect deformability. Hindawi Publishing Corporation 2014 2014-09-10 /pmc/articles/PMC4176636/ /pubmed/25295273 http://dx.doi.org/10.1155/2014/764268 Text en Copyright © 2014 Judith C. A. Cluitmans et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cluitmans, Judith C. A.
Chokkalingam, Venkatachalam
Janssen, Arno M.
Brock, Roland
Huck, Wilhelm T. S.
Bosman, Giel J. C. G. M.
Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title_full Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title_fullStr Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title_full_unstemmed Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title_short Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
title_sort alterations in red blood cell deformability during storage: a microfluidic approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176636/
https://www.ncbi.nlm.nih.gov/pubmed/25295273
http://dx.doi.org/10.1155/2014/764268
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