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Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach
Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. D...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176636/ https://www.ncbi.nlm.nih.gov/pubmed/25295273 http://dx.doi.org/10.1155/2014/764268 |
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author | Cluitmans, Judith C. A. Chokkalingam, Venkatachalam Janssen, Arno M. Brock, Roland Huck, Wilhelm T. S. Bosman, Giel J. C. G. M. |
author_facet | Cluitmans, Judith C. A. Chokkalingam, Venkatachalam Janssen, Arno M. Brock, Roland Huck, Wilhelm T. S. Bosman, Giel J. C. G. M. |
author_sort | Cluitmans, Judith C. A. |
collection | PubMed |
description | Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. During blood bank storage and in many pathophysiological conditions, RBC morphology changes, which has been suggested to be associated with decreased deformability and removal of RBC. While various techniques provide information on the rheological properties of stored RBCs, their clinical significance is controversial. We developed a microfluidic approach for evaluating RBC deformability in a physiologically meaningful and clinically significant manner. Unlike other techniques, our method enables a high-throughput determination of changes in deformation capacity to provide statistically significant data, while providing morphological information at the single-cell level. Our data show that, under conditions that closely mimic capillary dimensions and flow, the capacity to deform and the capacity to relax are not affected during storage in the blood bank. Our data also show that altered cell morphology by itself does not necessarily affect deformability. |
format | Online Article Text |
id | pubmed-4176636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41766362014-10-07 Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach Cluitmans, Judith C. A. Chokkalingam, Venkatachalam Janssen, Arno M. Brock, Roland Huck, Wilhelm T. S. Bosman, Giel J. C. G. M. Biomed Res Int Research Article Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, the combined result of properties of the membrane-cytoskeleton complex, the surface area-to-volume ratio, and the hemoglobin content, is a critical determinant of capillary blood flow. During blood bank storage and in many pathophysiological conditions, RBC morphology changes, which has been suggested to be associated with decreased deformability and removal of RBC. While various techniques provide information on the rheological properties of stored RBCs, their clinical significance is controversial. We developed a microfluidic approach for evaluating RBC deformability in a physiologically meaningful and clinically significant manner. Unlike other techniques, our method enables a high-throughput determination of changes in deformation capacity to provide statistically significant data, while providing morphological information at the single-cell level. Our data show that, under conditions that closely mimic capillary dimensions and flow, the capacity to deform and the capacity to relax are not affected during storage in the blood bank. Our data also show that altered cell morphology by itself does not necessarily affect deformability. Hindawi Publishing Corporation 2014 2014-09-10 /pmc/articles/PMC4176636/ /pubmed/25295273 http://dx.doi.org/10.1155/2014/764268 Text en Copyright © 2014 Judith C. A. Cluitmans et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cluitmans, Judith C. A. Chokkalingam, Venkatachalam Janssen, Arno M. Brock, Roland Huck, Wilhelm T. S. Bosman, Giel J. C. G. M. Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title | Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title_full | Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title_fullStr | Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title_full_unstemmed | Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title_short | Alterations in Red Blood Cell Deformability during Storage: A Microfluidic Approach |
title_sort | alterations in red blood cell deformability during storage: a microfluidic approach |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176636/ https://www.ncbi.nlm.nih.gov/pubmed/25295273 http://dx.doi.org/10.1155/2014/764268 |
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