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Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies

BACKGROUND: This study was conducted to determine the role of enteroaggregative Escherichia coli (EAEC) in inflammatory diarrhea among hospitalized patients in Kolkata. The inflammatory pathogenesis of EAEC was established in mice model and histopathological studies. Presence of fecal leucocytes (FL...

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Autores principales: Saha, Dhira Rani, Guin, Sucharita, Krishnan, Rajendran, Nag, Dhrubajyoti, Koley, Hemanta, Shinoda, Sumio, Ramamurthy, Thandavarayan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176733/
https://www.ncbi.nlm.nih.gov/pubmed/24294997
http://dx.doi.org/10.1186/1757-4749-5-36
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author Saha, Dhira Rani
Guin, Sucharita
Krishnan, Rajendran
Nag, Dhrubajyoti
Koley, Hemanta
Shinoda, Sumio
Ramamurthy, Thandavarayan
author_facet Saha, Dhira Rani
Guin, Sucharita
Krishnan, Rajendran
Nag, Dhrubajyoti
Koley, Hemanta
Shinoda, Sumio
Ramamurthy, Thandavarayan
author_sort Saha, Dhira Rani
collection PubMed
description BACKGROUND: This study was conducted to determine the role of enteroaggregative Escherichia coli (EAEC) in inflammatory diarrhea among hospitalized patients in Kolkata. The inflammatory pathogenesis of EAEC was established in mice model and histopathological studies. Presence of fecal leucocytes (FLCs) can be suspected for EAEC infection solely or as a mixed with other enteric pathogens. METHODS: Active surveillance was conducted for 2 years on 2 random days per week with every 5(th) patient admitted to the Infectious Diseases Hospital (IDH). Diarrheal samples were processed by conventional culture, microscopy, ELISA and molecular methods. Two EAEC isolated as sole pathogens were examined in mice after induced intestinal infection. The intestinal tissue samples were processed to analyze the histological changes. RESULTS: Of the 2519 samples screened, fecal leucocytes, erythrocytes and occult blood were detected in 1629 samples. Most of the patients had acute watery diarrhea (75%) and vomiting (78%). Vibrio cholerae O1 was the main pathogen in patients of 5–10 years age group (33%). Shigellosis was more in children from 2–5 years of age (19%), whereas children <2 years appeared to be susceptible for infection caused by EAEC (16%). When tested for the pathogenicity, the EAEC strains colonized well and caused inflammatory infection in the gut mucosa of BALB/C mice. CONCLUSION: This hospital-based surveillance revealed prevalence of large number of inflammatory diarrhea. EAEC was the suspected pathogen and <2 years children appeared to be the most susceptible age group. BALB/C mice may be a suitable animal model to study the EAEC-mediated pathogenesis.
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spelling pubmed-41767332014-09-28 Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies Saha, Dhira Rani Guin, Sucharita Krishnan, Rajendran Nag, Dhrubajyoti Koley, Hemanta Shinoda, Sumio Ramamurthy, Thandavarayan Gut Pathog Research BACKGROUND: This study was conducted to determine the role of enteroaggregative Escherichia coli (EAEC) in inflammatory diarrhea among hospitalized patients in Kolkata. The inflammatory pathogenesis of EAEC was established in mice model and histopathological studies. Presence of fecal leucocytes (FLCs) can be suspected for EAEC infection solely or as a mixed with other enteric pathogens. METHODS: Active surveillance was conducted for 2 years on 2 random days per week with every 5(th) patient admitted to the Infectious Diseases Hospital (IDH). Diarrheal samples were processed by conventional culture, microscopy, ELISA and molecular methods. Two EAEC isolated as sole pathogens were examined in mice after induced intestinal infection. The intestinal tissue samples were processed to analyze the histological changes. RESULTS: Of the 2519 samples screened, fecal leucocytes, erythrocytes and occult blood were detected in 1629 samples. Most of the patients had acute watery diarrhea (75%) and vomiting (78%). Vibrio cholerae O1 was the main pathogen in patients of 5–10 years age group (33%). Shigellosis was more in children from 2–5 years of age (19%), whereas children <2 years appeared to be susceptible for infection caused by EAEC (16%). When tested for the pathogenicity, the EAEC strains colonized well and caused inflammatory infection in the gut mucosa of BALB/C mice. CONCLUSION: This hospital-based surveillance revealed prevalence of large number of inflammatory diarrhea. EAEC was the suspected pathogen and <2 years children appeared to be the most susceptible age group. BALB/C mice may be a suitable animal model to study the EAEC-mediated pathogenesis. BioMed Central 2013-12-03 /pmc/articles/PMC4176733/ /pubmed/24294997 http://dx.doi.org/10.1186/1757-4749-5-36 Text en Copyright © 2013 Saha et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Saha, Dhira Rani
Guin, Sucharita
Krishnan, Rajendran
Nag, Dhrubajyoti
Koley, Hemanta
Shinoda, Sumio
Ramamurthy, Thandavarayan
Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title_full Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title_fullStr Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title_full_unstemmed Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title_short Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies
title_sort inflammatory diarrhea due to enteroaggregative escherichia coli: evidence from clinical and mice model studies
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176733/
https://www.ncbi.nlm.nih.gov/pubmed/24294997
http://dx.doi.org/10.1186/1757-4749-5-36
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