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Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep

BACKGROUND: It was recently shown that niacin supplementation counteracts the obesity-induced muscle fiber transition from oxidative type I to glycolytic type II and increases the number of type I fibers in skeletal muscle of obese Zucker rats. These effects were likely mediated by the induction of...

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Autores principales: Khan, Muckta, Couturier, Aline, Kubens, Johanna F, Most, Erika, Mooren, Frank-Christoph, Krüger, Karsten, Ringseis, Robert, Eder, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176759/
https://www.ncbi.nlm.nih.gov/pubmed/24267720
http://dx.doi.org/10.1186/1751-0147-55-85
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author Khan, Muckta
Couturier, Aline
Kubens, Johanna F
Most, Erika
Mooren, Frank-Christoph
Krüger, Karsten
Ringseis, Robert
Eder, Klaus
author_facet Khan, Muckta
Couturier, Aline
Kubens, Johanna F
Most, Erika
Mooren, Frank-Christoph
Krüger, Karsten
Ringseis, Robert
Eder, Klaus
author_sort Khan, Muckta
collection PubMed
description BACKGROUND: It was recently shown that niacin supplementation counteracts the obesity-induced muscle fiber transition from oxidative type I to glycolytic type II and increases the number of type I fibers in skeletal muscle of obese Zucker rats. These effects were likely mediated by the induction of key regulators of fiber transition, PPARδ (encoded by PPARD), PGC-1α (encoded by PPARGC1A) and PGC-1β (encoded by PPARGC1B), leading to type II to type I fiber transition and upregulation of genes involved in oxidative metabolism. The aim of the present study was to investigate whether niacin administration also influences fiber distribution and the metabolic phenotype of different muscles [M. longissimus dorsi (LD), M. semimembranosus (SM), M. semitendinosus (ST)] in sheep as a model for ruminants. For this purpose, 16 male, 11 wk old Rhoen sheep were randomly allocated to two groups of 8 sheep each administered either no (control group) or 1 g niacin per day (niacin group) for 4 wk. RESULTS: After 4 wk, the percentage number of type I fibers in LD, SM and ST muscles was greater in the niacin group, whereas the percentage number of type II fibers was less in niacin group than in the control group (P < 0.05). The mRNA levels of PPARGC1A, PPARGC1B, and PPARD and the relative mRNA levels of genes involved in mitochondrial fatty acid uptake (CPT1B, SLC25A20), tricarboxylic acid cycle (SDHA), mitochondrial respiratory chain (COX5A, COX6A1), and angiogenesis (VEGFA) in LD, SM and ST muscles were greater (P < 0.05) or tended to be greater (P < 0.15) in the niacin group than in the control group. CONCLUSIONS: The study shows that niacin supplementation induces muscle fiber transition from type II to type I, and thereby an oxidative metabolic phenotype of skeletal muscle in sheep as a model for ruminants. The enhanced capacity of skeletal muscle to utilize fatty acids in ruminants might be particularly useful during metabolic states in which fatty acids are excessively mobilized from adipose tissue, such as during the early lactating period in high producing cows.
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spelling pubmed-41767592014-09-28 Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep Khan, Muckta Couturier, Aline Kubens, Johanna F Most, Erika Mooren, Frank-Christoph Krüger, Karsten Ringseis, Robert Eder, Klaus Acta Vet Scand Research BACKGROUND: It was recently shown that niacin supplementation counteracts the obesity-induced muscle fiber transition from oxidative type I to glycolytic type II and increases the number of type I fibers in skeletal muscle of obese Zucker rats. These effects were likely mediated by the induction of key regulators of fiber transition, PPARδ (encoded by PPARD), PGC-1α (encoded by PPARGC1A) and PGC-1β (encoded by PPARGC1B), leading to type II to type I fiber transition and upregulation of genes involved in oxidative metabolism. The aim of the present study was to investigate whether niacin administration also influences fiber distribution and the metabolic phenotype of different muscles [M. longissimus dorsi (LD), M. semimembranosus (SM), M. semitendinosus (ST)] in sheep as a model for ruminants. For this purpose, 16 male, 11 wk old Rhoen sheep were randomly allocated to two groups of 8 sheep each administered either no (control group) or 1 g niacin per day (niacin group) for 4 wk. RESULTS: After 4 wk, the percentage number of type I fibers in LD, SM and ST muscles was greater in the niacin group, whereas the percentage number of type II fibers was less in niacin group than in the control group (P < 0.05). The mRNA levels of PPARGC1A, PPARGC1B, and PPARD and the relative mRNA levels of genes involved in mitochondrial fatty acid uptake (CPT1B, SLC25A20), tricarboxylic acid cycle (SDHA), mitochondrial respiratory chain (COX5A, COX6A1), and angiogenesis (VEGFA) in LD, SM and ST muscles were greater (P < 0.05) or tended to be greater (P < 0.15) in the niacin group than in the control group. CONCLUSIONS: The study shows that niacin supplementation induces muscle fiber transition from type II to type I, and thereby an oxidative metabolic phenotype of skeletal muscle in sheep as a model for ruminants. The enhanced capacity of skeletal muscle to utilize fatty acids in ruminants might be particularly useful during metabolic states in which fatty acids are excessively mobilized from adipose tissue, such as during the early lactating period in high producing cows. BioMed Central 2013-11-22 /pmc/articles/PMC4176759/ /pubmed/24267720 http://dx.doi.org/10.1186/1751-0147-55-85 Text en Copyright © 2013 Khan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Khan, Muckta
Couturier, Aline
Kubens, Johanna F
Most, Erika
Mooren, Frank-Christoph
Krüger, Karsten
Ringseis, Robert
Eder, Klaus
Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title_full Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title_fullStr Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title_full_unstemmed Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title_short Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
title_sort niacin supplementation induces type ii to type i muscle fiber transition in skeletal muscle of sheep
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176759/
https://www.ncbi.nlm.nih.gov/pubmed/24267720
http://dx.doi.org/10.1186/1751-0147-55-85
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