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Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection

BACKGROUND: Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthma...

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Autores principales: Giuffrida, María J, Valero, Nereida, Mosquera, Jesús, Alvarez de Mon, Melchor, Chacín, Betulio, Espina, Luz Marina, Gotera, Jennifer, Bermudez, John, Mavarez, Alibeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177805/
https://www.ncbi.nlm.nih.gov/pubmed/23962134
http://dx.doi.org/10.1111/irv.12155
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author Giuffrida, María J
Valero, Nereida
Mosquera, Jesús
Alvarez de Mon, Melchor
Chacín, Betulio
Espina, Luz Marina
Gotera, Jennifer
Bermudez, John
Mavarez, Alibeth
author_facet Giuffrida, María J
Valero, Nereida
Mosquera, Jesús
Alvarez de Mon, Melchor
Chacín, Betulio
Espina, Luz Marina
Gotera, Jennifer
Bermudez, John
Mavarez, Alibeth
author_sort Giuffrida, María J
collection PubMed
description BACKGROUND: Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients. OBJECTIVES: The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles. PATIENTS/METHODS: Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses. RESULTS: Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients. CONCLUSIONS: Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections.
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spelling pubmed-41778052014-10-29 Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection Giuffrida, María J Valero, Nereida Mosquera, Jesús Alvarez de Mon, Melchor Chacín, Betulio Espina, Luz Marina Gotera, Jennifer Bermudez, John Mavarez, Alibeth Influenza Other Respir Viruses Original Articles BACKGROUND: Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients. OBJECTIVES: The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles. PATIENTS/METHODS: Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses. RESULTS: Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients. CONCLUSIONS: Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections. Blackwell Publishing Ltd 2014-01 2013-08-21 /pmc/articles/PMC4177805/ /pubmed/23962134 http://dx.doi.org/10.1111/irv.12155 Text en © 2013 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Giuffrida, María J
Valero, Nereida
Mosquera, Jesús
Alvarez de Mon, Melchor
Chacín, Betulio
Espina, Luz Marina
Gotera, Jennifer
Bermudez, John
Mavarez, Alibeth
Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title_full Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title_fullStr Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title_full_unstemmed Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title_short Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
title_sort increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177805/
https://www.ncbi.nlm.nih.gov/pubmed/23962134
http://dx.doi.org/10.1111/irv.12155
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