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Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177895/ https://www.ncbi.nlm.nih.gov/pubmed/25254556 http://dx.doi.org/10.1371/journal.pone.0108680 |
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author | Tehseen, Muhammad Liao, Chunyan Dacres, Helen Dumancic, Mira Trowell, Stephen Anderson, Alisha |
author_facet | Tehseen, Muhammad Liao, Chunyan Dacres, Helen Dumancic, Mira Trowell, Stephen Anderson, Alisha |
author_sort | Tehseen, Muhammad |
collection | PubMed |
description | It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated. |
format | Online Article Text |
id | pubmed-4177895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41778952014-10-02 Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast Tehseen, Muhammad Liao, Chunyan Dacres, Helen Dumancic, Mira Trowell, Stephen Anderson, Alisha PLoS One Research Article It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated. Public Library of Science 2014-09-25 /pmc/articles/PMC4177895/ /pubmed/25254556 http://dx.doi.org/10.1371/journal.pone.0108680 Text en © 2014 Tehseen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tehseen, Muhammad Liao, Chunyan Dacres, Helen Dumancic, Mira Trowell, Stephen Anderson, Alisha Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title | Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title_full | Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title_fullStr | Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title_full_unstemmed | Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title_short | Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast |
title_sort | oligomerisation of c. elegans olfactory receptors, odr-10 and str-112, in yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177895/ https://www.ncbi.nlm.nih.gov/pubmed/25254556 http://dx.doi.org/10.1371/journal.pone.0108680 |
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